Unnoticed Spread of Class 1 Integrons in Gram‐Positive Clinical Strains Isolated in Guangzhou, China
Unnoticed Spread of Class 1 Integrons in Gram‐Positive Clinical Strains Isolated in Guangzhou, China
复制标题
DOI：
10.1111/j.1348-0421.2006.tb03815.x
复制
发表时间：
2006-06
影响因子：
2.6
通讯作者：
Lei Shi;Mei-Shu Zheng;Zenghuang Xiao;M. Asakura;Jianyu Su;Lin Li;S. Yamasaki
中科院分区：
文献类型：
--
作者：
Lei Shi;Mei-Shu Zheng;Zenghuang Xiao;M. Asakura;Jianyu Su;Lin Li;S. Yamasaki
采用intI1特异性引物对从中国采集的临床标本中分离出的46株革兰氏阳性菌进行PCR分析，并对intI1阳性菌株进一步进行耐药基因盒分析。所有分离株的基因组中都具有 1 类整合子，并且基因盒阵列为 dhfrXII-orfF-aadA2，这与其他生物体非常相似，除了一个分离株携带含有 aadA2 基因盒的 1 类整合子的额外副本。总而言之，结果表明 1 类整合子在中国广州分离的革兰氏阳性临床菌株中广泛存在。
A total of 46 gram‐positive bacteria isolated from clinical specimens collected in China were subjected to PCR analysis with the intI1‐specific primers, and the intI1‐positive strains were further analyzed for their resistance gene cassette. All isolates possessed the class 1 integron in their genomes and the array of gene cassettes was dhfrXII‐orfF‐aadA2, which is very similar to other organisms except in one isolate carrying an additional copy of the class 1 integron containing the aadA2 gene cassette. Altogether, the results indicate that the class 1 integron is widespread in gram‐positive clinical strains isolated in Guangzhou, China.