Do results of the T-SPOT.TB interferon-γ release assay change after treatment of tuberculosis?
Do results of the T-SPOT.TB interferon-γ release assay change after treatment of tuberculosis?
复制标题
DOI：
10.1016/j.rmed.2008.09.012
复制
发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Janssens, Jean-Paul
中科院分区：
文献类型：
--
作者：
Bosshard, Valerie;Roux-Lombard, Pascale;Janssens, Jean-Paul
背景：暴露于结核分枝杆菌特异性抗原的淋巴细胞产生的干扰素 (IFN-gamma) 已被证明与抗原载量和疾病活动相关。研究目的：确定结核病 (TB) 治疗是否导致 IFN-gamma 释放测定（T-SPOT.TB，牛津免疫技术公司，英国）结果的减少和/或逆转，从而确定 T-SPOT.TB 是否可用于监测对结核病的反应。方法：对 HIV 阴性结核病患者的 T-SPOT.TB (R) 进行定性和定量分析（SFU：斑点形成单位），治疗最初 2 周 (T0)、治疗结束时 (TE) 和 6 个月后 (TE + 6)。结果：T0 时平均 SFU (SD) 为 75 (58；n = 62)，TE 时为 46 (55；n = 55)， 33 (46；n = 41)，TE + 6；阳性率分别为98%、93%和98%。 SFU（配对样本，n = 36）在 T0 和 TE 之间显着下降； T0 和 TE 之间发生 2 例逆转 (6%)，但 TE 和 TE + 6 之间没有发生逆转。在 T0 和 TE 之间 SFU 增加的 6 名患者 (17%) 中，5 名在 TE 和 TE + 6 时获得良好结果。结论：治疗中 SFU 的减少表明与抗原蟾蜍有关；然而，持续的高 SFU 并不能预测不良结果，并且测试逆转的情况很少见。该试验已在 www.clinicaltrials.gov (NCT00595907) 上注册。 (C) 2008 Elsevier Ltd. 保留所有权利。
Background: Interferon-gamma (IFN-gamma) production by lymphocytes exposed to antigens specific of Mycobacterium tuberculosis has been shown to correlate with antigen load and disease activity.Aim of study: To determine whether treatment of tuberculosis (TB) led to a decrease and/or a reversion of results of a IFN-gamma release assay (T-SPOT.TB, Oxford Immunotec, UK) and thus if T-SPOT.TB could be used to monitor response to treatment.Methods: Qualitative and quantitative analysis (SFUs: spot-forming units) of T-SPOT.TB (R) in HIV-negative patients with TB, during initial 2 weeks of treatment (T0), at end of treatment (TE) and 6 months later (TE + 6).Results: Mean SFU (SD) was 75 (58; n = 62) at T0, 46 (55; n = 55) at TE, and 33 (46; n = 41) at TE + 6; positive rate was 98%, 93% and 98%, respectively. SFUs (paired samples, n = 36) decreased significantly between T0 and TE; 2 reversions occurred between T0 and TE (6%), but none between TE and TE + 6. Of 6 patients (17%) with an increase in SFUs between T0 and TE, 5 had a favourable outcome at TE and TE + 6.Conclusion: Decrease in SFUs under treatment suggests a relationship with antigen toad; however, persisting high SFUs were not predictive of unfavourable outcome and test reversion was rare. This trial was registered at www.clinicaltrials.gov (NCT00595907). (C) 2008 Elsevier Ltd. All rights reserved.