Ultraviolet action spectrum for intracellular free Ca2+ increase in human epidermal keratinocytes.
Ultraviolet action spectrum for intracellular free Ca2+ increase in human epidermal keratinocytes.
复制标题
人表皮角质形成细胞中细胞内游离 Ca2 增加的紫外线作用光谱。
作者：
T. Nakagaki;J. Oda;H. Koizumi;T. Fukaya;C. Yasui;T. Ueda
应用荧光比率成像技术（Fura-2-AM）测定细胞内游离钙浓度（[Ca ~（2+）]i），研究紫外线（UV）对正常人表皮角质形成细胞的影响。紫外光照射后，[Ca ~（2+）]i在一定的滞后时间后急剧增加，且在较低温度下紫外光敏感性较高。统计学上，[Ca 2 +]i的分布随着平均值变大而变宽，并且在研究的所有波长（200-400 nm）下，受影响细胞的数量在超过一定通量（光强度x时间[光子/cm 2]）时急剧增加。其作用光谱在230 nm左右为一个单峰，向长波紫外区逐渐减弱。
Effects of UV on normal human epidermal keratinocytes were studied by measuring the intracellular free Ca2+ concentration ([Ca2+]i) using fluorescence ratio imaging (fura-2-AM). Upon UV irradiation the [Ca2+]i increased sharply after a certain lag time, and the UV sensitivity was higher at lower temperatures. Statistically the distribution of [Ca2+]i became broader as the mean values became larger, and the number of affected cells increased sharply above a certain fluence (light intensity x time [photons/cm2]) at all wavelengths studied (200-400 nm). The action spectrum showed a single peak at about 230 nm and decreased gradually toward longer-wavelength UV regions.