Genomic signature and mutation trend analysis of pandemic (H1N1) 2009 influenza A virus.
Genomic signature and mutation trend analysis of pandemic (H1N1) 2009 influenza A virus.
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2009 年 H1N1 甲型流感病毒的基因组特征和突变趋势分析
DOI：
10.1371/journal.pone.0009549
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发表时间：
2010-03-08
期刊：
影响因子：
3.7
通讯作者：
Jiang S
中科院分区：
文献类型：
--
作者：
Pan C;Cheung B;Tan S;Li C;Li L;Liu S;Jiang S
一种新型猪源性甲型H1N1流感病毒（H1N1 pdm，也称为S-OIV）被确定为21世纪世纪第一次流感大流行的病原体，但尚未确定赋予其人传人能力的分子特征。本文将2009年H1N1 pdm毒株与其他大流行毒株及人、猪、禽分离株的蛋白质序列进行了比较，分析了2009年H1N1 pdm毒株蛋白质中与种属和非种属相关的特征位点和非特征位点残基的突变趋势。我们证实，2009年H1N1 pdm的宿主特异性基因组特征（主要是猪样的）与1918年H1N1 pdm的宿主特异性基因组特征高度相同。在大流行警戒级别从4级提高到6级的短时间内，NP-100位置上的一个特征残基从缬氨酸突变为异亮氨酸。在2009年的流行期间，在位置NA-91、NA-233、HA-206和NS 1 -123处的四个非特征残基也发生了变化。除NA-91外，所有突变残基均位于病毒功能区，提示它们可能在2009 H1N1 pdm的人类适应性和毒力中发挥作用。
A novel swine-origin pandemic influenza A(H1N1) virus (H1N1pdm, also referred to as S-OIV) was identified as the causative agent of the 21st century's first influenza pandemic, but molecular features conferring its ability of human-to-human transmission has not been identified. Here we compared the protein sequences of 2009 H1N1pdm strains with those causing other pandemics and the viruses isolated from humans, swines and avians, and then analyzed the mutation trend of the residues at the signature and non-signature positions, which are species- and non-species-associated, respectively, in the proteins of H1N1pdm during the pandemic of 2009. We confirmed that the host-specific genomic signatures of 2009 H1N1pdm, which are mainly swine-like, were highly identical to those of the 1918 H1N1pdm. During the short period of time when the pandemic alert level was raised from phase 4 to phase 6, one signature residue at the position of NP-100 mutated from valine to isoleucine. Four non-signature residues, at positions NA-91, NA-233, HA-206, and NS1-123, also changed during the epidemic in 2009. All these mutant residues, except that at NA-91, are located in the viral functional domains, suggesting that they may play roles in the human adaption and virulence of 2009 H1N1pdm.
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影响因子：
64.8
作者：
Collins, Patrick J.;Haire, Lesley F.;Gamblin, Steven J.
通讯作者：
Gamblin, Steven J.
影响因子：
6.4
作者：
Gubareva, LV;Kaiser, L;Hayden, FG
通讯作者：
Hayden, FG
DOI：
10.1073/pnas.0507415102
发表时间：
2005-12-20
影响因子：
11.1
作者：
Gabriel, G;Dauber, B;Stech, J
通讯作者：
Stech, J
影响因子：
5.4
作者：
Talon, J;Horvath, CM;García-Sastre, A
通讯作者：
García-Sastre, A
影响因子：
4.1
作者：
Vana, Geoff;Westover, Kristi M.
通讯作者：
Westover, Kristi M.