The synthesis and secretion of key substances in the flavonoid metabolic pathway responding to different nitrogen sources during early growth stages in Robinia pseudoacacia
The synthesis and secretion of key substances in the flavonoid metabolic pathway responding to different nitrogen sources during early growth stages in Robinia pseudoacacia
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DOI：
10.1007/s11104-023-06286-y
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发表时间：
2023-09-19
期刊：
影响因子：
4.9
通讯作者：
Chou，Minxia
中科院分区：
文献类型：
--
作者：
Li，Yuanli;Shen，Yongrui;Chou，Minxia
Background and aimsLegumes can obtain nitrogen from mineral nitrogen and symbiotic nitrogen; however, the regulatory mechanisms by which legume plants respond to various nitrogen sources are still unclear.MethodsHere, the flavonoid metabolism profiles ofRobinia pseudoacacia(black locust) in response to different nitrogen sources during the early growth stages were performed using the hydroponics method.ResultsThe flavonoids were accumulated specifically responding to different nitrogen sources (symbiotic nitrogen and NO3−) in the roots and root exudates ofR. pseudoacaciaand showed growth-stage-dependent changes in the plant growth process. More flavonoids were synthesized and secreted under nitrogen deficiency. The differential flavonoids among different treatments were significantly enriched in the flavone and flavonol biosynthesis pathway and isoflavonoid biosynthesis pathway. Liquiritigenin, naringenin, apigenin and kaempferol were not only the metabolic nodes of these two metabolic pathways but also the key nodes in the differential flavonoid network. Therefore, these four flavonoids may be the key flavonoids ofR. pseudoacaciaresponding to different nitrogen sources.ConclusionOur findings provide an important insight into the adaptability of legumes to different nitrogen environments and are of great significance for understanding the molecular mechanisms underlying nitrogen utilization in legumes and will contribute to the development of sustainable agricultural practices and crop production.
