Down-regulation of sarcolipin mRNA expression in chronic atrial fibrillation
Down-regulation of sarcolipin mRNA expression in chronic atrial fibrillation
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DOI：
10.1111/j.1365-2362.2004.01422.x
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发表时间：
2004-11-01
影响因子：
5.5
通讯作者：
Ishikawa, Y
中科院分区：
文献类型：
--
作者：
Uemura, N;Ohkusa, T;Ishikawa, Y
背景细胞内钙稳态异常是慢性心房颤动的重要调节因子。Sarcolipin是受磷蛋白的同源物，在心房中特异性表达，并且可能在调节心房中细胞内Ca 2+稳态中发挥重要作用。为探讨慢性心房颤动（房颤）患者心房肌中sarcolipin mRNA的表达，采用免疫组织化学方法检测9例二尖瓣病变伴房颤（MVD/AF）患者、9例MVD伴正常窦性心律（MVD/NSR）患者、10例正常窦性心律（MVD/NSR）患者、10例正常窦性心律（MVD/NSR）患者右心房肌中sarcolipin、phospholamban、cardiac calsequestrin和sodium calcium exchange mRNAs的表达。10例正常窦性心律的心内直视手术患者（对照组）。使用ABI PRISM 7700序列检测系统测量mRNA的表达结果在MVD/AF中，肌磷脂mRNA的相对表达水平显著低于MVD/AF组，MVD/NSR组（1.28 +/-0.17，P < 0.01）和对照组（1.10 +/- 0.10，P <0.05）之间差异无统计学意义（P> 0.05）。高右房压组心肌肌磷脂mRNA表达水平明显低于低右房压组。受磷蛋白、心肌钙螯合素和钠钙交换蛋白mRNA的表达水平在3组间具有可比性。结论慢性电负荷和机械负荷均降低慢性房颤患者右心房肌磷脂mRNA的表达。Sarcolipin mRNA的下调可能是心房颤动诱导的心房重构的一部分。
Background Abnormal intracellular Ca2+ homeostasis is an important modulator of chronic atrial fibrillation. Sarcolipin, a homologue of phospholamban, is specifically expressed in the atria, and may play an important role in modulating intracellular Ca2+ homeostasis in the atria. The aim of this study was to investigate the expression of sarcolipin mRNA in the atrial myocardium of patients with chronic atrial fibrillation.Methods We analyzed the expression of sarcolipin, phospholamban, cardiac calsequestrin and sodium calcium exchanger mRNAs in the right atrial myocardium from nine patients with mitral valvular disease with atrial fibrillation (MVD/AF), nine patients with MVD who had normal sinus rhythm (MVD/NSR), and 10 control patients with normal sinus rhythm who received open heart surgery (controls). The expression of mRNA was measured using the ABI PRISM 7700 Sequence Detection System (Applied Biosystems, Foster City, CA).Results Relative expression levels of sarcolipin mRNA were significantly lower in MVD/AF (0.60 +/- 0.11) than in either MVD/NSR (1.28 +/- 0.17, P < 0.01) or controls (1.10 +/- 0.10, P < 0.05). The expression levels of sarcolipin mRNA were significantly lower in the group with high values for right atrial pressure. The expression levels of phospholamban, cardiac calsequestrin and sodium calcium exchanger mRNAs were comparable among all three groups.Conclusions Chronic electrical and mechanical overload decreased the expression of sarcolipin mRNA in the right atrial myocardium in patients with chronic atrial fibrillation. Down-regulation of sarcolipin mRNA may be part of atrial fibrillation-induced atrial remodelling.