Design and Development of IKZF2 and CK1α Dual Degraders.
Design and Development of IKZF2 and CK1α Dual Degraders.
复制标题
IKZF2 和 CK1α 双降解器的设计和开发。
DOI：
10.1021/acs.jmedchem.3c01736
复制
发表时间：
2023
影响因子：
7.3
通讯作者：
Woo,ChristinaM
中科院分区：
文献类型：
--
作者：
Miyamoto,DavidK;Curnutt,NicoleM;Park,Sun-Mi;Stavropoulos,Alexios;Kharas,MichaelG;Woo,ChristinaM
Lenalidomide achieves its therapeutic efficacy by recruiting and removing proteins of therapeutic interest through the E3 ligase substrate adapter cereblon. Here, we report the design and characterization of 81 cereblon ligands for their ability to degrade the transcription factor Helios (IKZF2) and casein kinase 1 alpha (CK1α). We identified a key naphthamide scaffold that depleted both intended targets in acute myeloid leukemia MOLM-13 cells. Structure–activity relationship studies for degradation of the desired targets over other targets (IKZF1, GSPT1) afforded an initial lead compoundDEG-35. A subsequent scaffold replacement campaign identifiedDEG-77, which selectively degrades IKZF2 and CK1α, and possesses suitable pharmacokinetic properties, solubility, and selectivity for in vivo studies. Finally, we show thatDEG-77has antiproliferative activity in the diffuse large B cell lymphoma cell line OCI-LY3 and the ovarian cancer cell line A2780 indicating that the dual degrader strategy may have efficacy against additional types of cancer.
