Quantification of hemolymph proteins as a fast method for testing protein diets for honey bees (Hymenoptera: Apidae)
Quantification of hemolymph proteins as a fast method for testing protein diets for honey bees (Hymenoptera: Apidae)
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DOI：
10.1093/jee/91.6.1284
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发表时间：
1998-12-01
影响因子：
2.2
通讯作者：
Bitondi, MMG
中科院分区：
文献类型：
--
作者：
Cremonez, TM;De Jong, D;Bitondi, MMG
通过对蜜蜂血淋巴蛋白质的测定，确定了蛋白质饲料作为花粉替代品的有效性。将120只新出现的工蜂饲养在实验室的小笼子里，从成蜂羽化到6d后，用蜂面包或未加工的花粉(天然蛋白质饲料)、大豆/酵母或玉米粉(替代蛋白质饲料)或蔗糖溶液(非蛋白质饲料)喂养。在成年后第0、2、4、6天测定血淋巴中蛋白质的含量。此外，通过火箭免疫电泳法对6天龄蜜蜂的血淋巴进行了卵黄蛋白原(年轻成年工蜂的一种主要蛋白质)效价的测定。在以蜂粮、大豆/酵母或花粉为食的蜜蜂血淋巴中，从成虫0天到第6天，蛋白质滴度有显著的递增趋势。然而，在只喂食玉米粉或蔗糖的蜜蜂中，蛋白质显著减少。6日龄蜜蜂的蛋白质效价对不同日粮的区分能力最强。当比较不同的饲料时，6日龄蜜蜂的蛋白质效价与卵黄蛋白原水平高度相关。蛋白质值反映的是饲料中蛋白质的数量和可用性，而不是消耗量，这与所有使用的蛋白质饲料相似。总蛋白质测定和卵黄蛋白原水平测定都被证明是比较蛋白质饮食有效性的客观方法；但前者更快、更便宜，可以很容易地用于常规分析。
Hemolymph protein measurements In honey bees, Apis mellifera L., were used to determine the efficiency of protein diets as pollen substitutes. Groups of 120 newly emerged worker bees were kept in small cages in the laboratory and fed on bee bread or unprocessed pollen (natural protein diets), soybean/yeast or corn meal (alternative protein diets), or a sucrose solution (nonprotein diet), from adult emergence until 6 d later. The protein content in hemolymph was determined in these bees at 0, 2, 4, and 6 d of adult life. Additionally, vitellogenin (a major protein in young adult worker bees) titer was measured through rocket immunoelectrophoresis of the hemolymph of 6-d-old bees. A significant and progressive rise in protein titers was observed from 0 to the 6th d of adult life in the hemolymph of bees fed on bee bread, soybean/yeast, or pollen. However, a significant protein reduction was recorded in bees fed on corn meal or sucrose only. The protein titer of 6-d-old bees gave the best discrimination between diets. Protein titers were highly correlated with vitellogenin levels in 6-d-old bees, when the different diets were compared. The protein values reflected the quantity and usability of the protein in the diets and not the consumption, which was similar for all protein diets used. Both total protein measurement and vitellogenin level determination proved to be objective methods for comparing the effectiveness of protein diets; however, the former is faster and less expensive and could easily be used for routine analyses.