Organic nitrogen rearranges both structure and activity of the soil-borne microbial seedbank.
Organic nitrogen rearranges both structure and activity of the soil-borne microbial seedbank.
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DOI：
10.1038/srep42634
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发表时间：
2017-02-15
影响因子：
4.6
通讯作者：
Kuramae EE
中科院分区：
文献类型：
--
作者：
Leite MF;Pan Y;Bloem J;Berge HT;Kuramae EE
使用有机改良剂对于作物生产来说是一种有价值的策略。然而，目前尚不清楚有机改良剂如何塑造土壤微生物群落结构和活动，以及这些变化如何影响养分矿化率。我们在中生态研究中评估了各种有机改良剂（碳/氮 (C/N) 比和可降解性）对土壤微生物群的影响，时间为 32、69 和 132 天。收集土壤样本以确定群落结构（通过 16S 和 18S rRNA 基因序列评估）、微生物生物量（真菌和细菌）、微生物活性（亮氨酸掺入和活性菌丝长度）以及碳和氮矿化率。我们将微生物土壤 DNA 视为微生物种子库。高 C/N 比有利于真菌的存在，而低 C/N 则有利于细菌种群的优势。我们的结果表明，有机改良剂通过反馈机制塑造土壤微生物群落结构，微生物活动通过反馈机制响应不断变化的有机输入并重新排列微生物种子库的组成。我们假设微生物种子库的组成根据个体物种的抵抗力和恢复力对有机投入的变化做出反应，而微生物活动的变化可能导致影响植物养分吸收的各种土壤养分的可用性增加或减少。
Use of organic amendments is a valuable strategy for crop production. However, it remains unclear how organic amendments shape both soil microbial community structure and activity, and how these changes impact nutrient mineralization rates. We evaluated the effect of various organic amendments, which range in Carbon/Nitrogen (C/N) ratio and degradability, on the soil microbiome in a mesocosm study at 32, 69 and 132 days. Soil samples were collected to determine community structure (assessed by 16S and 18S rRNA gene sequences), microbial biomass (fungi and bacteria), microbial activity (leucine incorporation and active hyphal length), and carbon and nitrogen mineralization rates. We considered the microbial soil DNA as the microbial seedbank. High C/N ratio favored fungal presence, while low C/N favored dominance of bacterial populations. Our results suggest that organic amendments shape the soil microbial community structure through a feedback mechanism by which microbial activity responds to changing organic inputs and rearranges composition of the microbial seedbank. We hypothesize that the microbial seedbank composition responds to changing organic inputs according to the resistance and resilience of individual species, while changes in microbial activity may result in increases or decreases in availability of various soil nutrients that affect plant nutrient uptake.