CAV-2-why a canine virus is a neurobiologist's best friend
CAV-2-why a canine virus is a neurobiologist's best friend
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DOI：
10.1016/j.coph.2015.08.004
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发表时间：
2015-10-01
影响因子：
4
通讯作者：
Kremer, Eric J.
中科院分区：
文献类型：
--
作者：
Junyent, Felix;Kremer, Eric J.
犬腺病毒2型（CAV-2）载体由于其可忽略的免疫原性、优先转导神经元、通过轴突运输广泛分布以及在哺乳动物脑中表达的持续时间而成为基础和应用神经生物学的有力工具。CAV-2载体通过选择性使用位于神经元突触前的柯萨奇病毒和腺病毒受体（CAR）而内化于神经元中。神经元内化和轴突运输由CAR介导，这增强了载体的生物分布。上述特征，以及辅助依赖性（HD）CAV-2载体的类似于30 kb的克隆能力，优化的CAV-2载体产生、生产和纯化，正在扩大CNS基因转移的治疗和基本选择。
Canine adenovirus type 2 (CAV-2) vectors are powerful tools for fundamental and applied neurobiology due to their negligible immunogenicity, preferential transduction of neurons, widespread distribution via axonal transport, and duration of expression in the mammalian brain. CAV-2 vectors are internalized in neurons by the selective use of coxsackievirus and adenovirus receptor (CAR), which is located at the presynapse in neurons. Neuronal internalization and axonal transport is mediated by CAR, which potentiates vector biodistribution. The above characteristics, together with the similar to 30 kb cloning capacity of helper-dependent (HD) CAV-2 vectors, optimized CAV-2 vector creation, production and purification, is expanding the therapeutic and fundamental options for CNS gene transfer.