Bioguided Fraction and Isolation of the Antitumor Components from Phyllanthus niruri L.
Bioguided Fraction and Isolation of the Antitumor Components from Phyllanthus niruri L.
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珍珠草中抗肿瘤成分的生物引导分离和分离。
DOI：
10.1155/2016/9729275
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发表时间：
2016
影响因子：
--
通讯作者：
Ming YL
中科院分区：
文献类型：
--
作者：
Zheng ZZ;Chen LH;Liu SS;Deng Y;Zheng GH;Gu Y;Ming YL
叶下珠，是一种著名的药用植物，在世界范围内被用作民间抗肿瘤药物。然而，P. niruri中的抗肿瘤成分尚未见报道。为了验证白花蛇舌草的抗肿瘤活性成分以及这些成分含量高的植物，我们采用不同的色谱方法从白花蛇舌草的乙酸乙酯部位中分离出抗肿瘤活性成分，通过1H-NMR、13 C-NMR、2D-NMR和质谱分析鉴定了它们为短叶苏木素羧酸乙酯和柯里拉京。细胞毒性实验表明柯里拉京具有广谱抗肿瘤活性，对正常细胞的毒性较低，具有较好的抗肿瘤潜力。10 μM柯里拉京与20 μM cDDP的药物相互作用系数（CDI）达0.77，表明柯里拉京对cDDP的抗肿瘤活性有促进作用。通过对10种已知含有柯里拉京的植物进行筛选，发现龙眼（Dimocarpus longan Lour.）柯里拉京含量最高。综上所述，柯里拉京是白花委陵菜中抗肿瘤的主要活性成分。L.在肝癌细胞中D.龙眼。
Phyllanthus niruri L., a well-known medicinal plant, has been used as a folk antitumor remedy in the worldwide scale. However, the antitumor components in P. niruri have not been reported. In order to verify the antitumor components of P. niruri and the plants which have the high content of these components, we isolated the antitumor components with bioguided fraction and isolation, by different chromatographic methods from the ethyl acetate fraction of P. niruri., and identified them as ethyl brevifolincarboxylate and corilagin by 1H-NMR, 13C-NMR, 2D-NMR, and mass spectrometric analyses. Cell cytotoxicity assays showed that corilagin has broad-spectrum antitumor activity, a better antitumor potential, and lower toxicity in normal cells. Besides, the coefficient of drug interaction (CDI) of 10 μM corilagin and 20 μM cDDP reached up to 0.77, which means corilagin can promote the antitumor activity of cDDP. Furthermore, by the extensive screening among 10 species of plants reported to contain corilagin, we found that Dimocarpus longan Lour. has the maximum content of corilagin. In conclusion, corilagin is the major active antitumor composition in P. niruri. L. on HCC cells and has high content in D. longan.