ELECTRON MICROSCOPY OF MYCOPLASMA GALLISEPTICUM AND MYCOPLASMA MYCOIDES USING NEGATIVE STAINING TECHNIQUE AND THEIR COMPARISON WITH MYXOVIRUS
ELECTRON MICROSCOPY OF MYCOPLASMA GALLISEPTICUM AND MYCOPLASMA MYCOIDES USING NEGATIVE STAINING TECHNIQUE AND THEIR COMPARISON WITH MYXOVIRUS
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DOI：
10.1111/j.1749-6632.1967.tb27658.x
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发表时间：
1967-01-01
影响因子：
5.2
通讯作者：
HORNE, RW
中科院分区：
文献类型：
--
作者：
CHU, HP;HORNE, RW
Materials and MethodsStrains of Organisms Used. Several strains of M. gallisepticum have been used but most of the work was done with strain 514 which was isolated from a case of fowl coryza in England in 1959 and has been through at least 100 passages in artificial media. Two strains of M. mycoides, var mycoides, one obtained from Dr. Klieneberger and the other from Dr. Freundt, and two strains of M. mycoides, var Capri, one isolated by Chu in 1949 (strain Ankara/49) and the other by Pillai in 1961 (strain Sudan G1/61) were used. One strain of nonpathogenic avian mycoplasma, A36, and one strain of Mycoplasma laidlawii were included for comparison. The strain of Newcastle Disease virus used was the Hertfordshire strain obtained from Weybridge Laboratory, UK Growth of the Organisms. The mycoplasmas were grown in liquid medium containing dialysable ingredients of yeast autolysate and peptone with the addition of 15% horse serum and 0.2% glucose (Chu and Newnham, 1959). Thallium Acetate (1/8000) and penicillin (100 iu/ml) and Andrades indicator were included in the medium. The complete medium (pH 7.2) was sterilized by passing it through a Seitz EK filter. This also served to remove any protein or other precipitates present in the medium. The medium was dispensed in 200 ml amount
