Comparison of Normalization Methods for Construction of Large, Multiplex Amplicon Pools for Next-Generation Sequencing
Comparison of Normalization Methods for Construction of Large, Multiplex Amplicon Pools for Next-Generation Sequencing
复制标题
DOI：
10.1128/aem.02585-09
复制
发表时间：
2010-06-01
影响因子：
4.4
通讯作者：
Spear, John R.
中科院分区：
文献类型：
--
作者：
Harris, J. Kirk;Sahl, Jason W.;Spear, John R.
构建用于测序的标记或条形码DNA的混合物是在每个样品需要有限序列数据的应用中有效使用下一代测序仪的重要要求。在许多应用中，下一代测序可以有效地用于对大量混合样品进行测序;一个例子是微生物群落的表征，其中
Constructing mixtures of tagged or bar-coded DNAs for sequencing is an important requirement for the efficient use of next-generation sequencers in applications where limited sequence data are required per sample. There are many applications in which next-generation sequencing can be used effectively to sequence large mixed samples; an example is the characterization of microbial communities where