Fluorescent Glyco Single-Chain Nanoparticle-Decorated Nanodiamonds.
Fluorescent Glyco Single-Chain Nanoparticle-Decorated Nanodiamonds.
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DOI：
10.1021/acsmacrolett.7b00659
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发表时间：
2017-10
影响因子：
5.8
通讯作者：
Kilian N. R. Wuest;Hongxu Lu;Donald S. Thomas;Anja S. Goldmann;M. Stenzel;C. Barner‐Kowollik
中科院分区：
文献类型：
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作者：
Kilian N. R. Wuest;Hongxu Lu;Donald S. Thomas;Anja S. Goldmann;M. Stenzel;C. Barner‐Kowollik
我们介绍了单糖链在水中的光诱导坍塌，产生了荧光糖基单链纳米颗粒(SCNPs)及其随后在纳米钻石上的功能化。糖共聚物前体是通过聚合乙酰化的甘露糖基甲基丙烯酸酯单体，然后经过脱保护和后聚合官能化步骤，引入前荧光光活性四唑基团和呋喃保护的马来酰亚胺基团而制备的。随后在高度稀释的水溶液中进行紫外光照射，触发分子内四唑介导的环加成反应，生成具有荧光和凝集素结合特性的糖基SCNPs。通过吸附将得到的SCNPs包覆在纳米钻石上，并对得到的杂化纳米颗粒的尺寸、功能和生物活性进行了深入的表征。通过改变SCNP的浓度可以获得不同的涂层密度。制备的纳米颗粒对小鼠RAW 264.7巨噬细胞无毒。此外，SCNPs的荧光可以通过共聚焦荧光显微镜在巨噬细胞中对SCNP包裹的纳米钻石进行成像。
We introduce the light-induced collapse of single glycopolymer chains in water generating fluorescent glyco single-chain nanoparticles (SCNPs) and their subsequent functionalization onto nanodiamonds. The glycopolymer precursors are prepared by polymerizing an acetylated mannose-based methacrylate monomer followed by a deprotection and postpolymerization functionalization step, introducing profluorescent photoactive tetrazole groups and furan-protected maleimide moieties. Subsequent UV irradiation in highly diluted aqueous solution triggers intramolecular tetrazole-mediated cycloadditions, yielding glyco SCNPs featuring fluorescence as well as lectin binding properties. The obtained SCNPs are coated onto nanodiamonds by adsorption, and the obtained hybrid nanoparticles are in depth characterized in terms of size, functionality, and bioactivity. Different coating densities are achieved by altering the SCNP concentration. The prepared nanoparticles are nontoxic in mouse RAW 264.7 macrophages. Furthermore, the fluorescence of the SCNPs can be exploited to image the SCNP-coated nanodiamonds in macrophage cells via confocal fluorescence microscopy.