The high-affinity binding of laminin to cells. Assignation of a major cell-binding site to the long arm of laminin and of a latent cell-binding site to its short arms.
The high-affinity binding of laminin to cells. Assignation of a major cell-binding site to the long arm of laminin and of a latent cell-binding site to its short arms.
复制标题
层粘连蛋白与细胞的高亲和力结合。
DOI：
--
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
D. Edgar
中科院分区：
文献类型：
--
作者：
V. Nurcombe;M. Aumailley;R. Timpl;D. Edgar
层粘连蛋白蛋白水解片段1（来自十字形层粘连蛋白分子短臂的交叉点）和8（来自层粘连蛋白长臂）与HT-1080人纤维肉瘤细胞上的不同受体结合;此处显示两个片段均抑制层粘连蛋白与这些细胞的高亲和力结合。片段8和层粘连蛋白之间的结合抑制是竞争性的，而片段1和层粘连蛋白之间的抑制是非竞争性的。这表明层粘连蛋白和片段8最可能共享相同的细胞受体，而层粘连蛋白和片段1结合不同的受体，抑制是由于空间位阻。令人惊讶的是，片段1-4（对应于层粘连蛋白的完整短臂）既不与HT-1080细胞结合，也不抑制层粘连蛋白或片段1的结合。然而，在用胃蛋白酶处理片段1-4后，释放出较小的亚片段1，其然后可以结合到细胞上，并且因此显示阻断层粘连蛋白和片段1的结合。我们的结论是，天然层粘连蛋白结合HT-1080细胞通过片段-8-结合位点附近的长臂末端。虽然这些细胞也有不同的受体短臂片段1，这个受体结合位点没有使用，因为它似乎是潜伏在天然层粘连蛋白分子。
The laminin proteolytic fragments 1 (derived from the intersection of the short arms of the cruciform laminin molecule) and 8 (derived from the laminin long arm) bind to distinct receptors on HT-1080 human fibrosarcoma cells; both fragments are shown here to inhibit the high-affinity binding of laminin to these cells. Inhibition of binding between fragment 8 and laminin was competitive, whereas that between fragment 1 and laminin was noncompetitive. This indicates that laminin and fragment 8 most probably share the same cellular receptors, whereas laminin and fragment 1 bind to distinct receptors, inhibition being due to steric hindrance. Surprisingly, fragment 1-4 (corresponding to the complete short arms of laminin) neither bound to HT-1080 cells nor inhibited the binding of laminin or fragment 1. After treatment of fragment 1-4 with pepsin, however, the smaller subfragment 1 was liberated, which could then bind to the cells, and so was shown to block the binding of laminin and fragment 1. We conclude that native laminin bound to HT-1080 cells via the fragment-8-binding site near the end of its long arm. Although these cells also have distinct receptors for the short arm fragment 1, this receptor-binding site was not used as it appeared to be latent within the native laminin molecule.
DOI：
10.1016/s0021-9258(18)89291-2
发表时间：
1985-04
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
S. K. Akiyama;K. Yamada
通讯作者：
S. K. Akiyama;K. Yamada
有证据表明精氨酰-甘氨酰-天冬氨酸肽和纤维蛋白原γ链肽在血小板上具有共同的结合位点。
DOI：
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发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lam,SC;Plow,EF;Smith,MA;Andrieux,A;Ryckwaert,JJ;Marguerie,G;Ginsberg,MH
通讯作者：
Ginsberg,MH