Astrocyte-specific expression of aquaporin-9 in mouse brain is increased after transient focal cerebral ischemia
Astrocyte-specific expression of aquaporin-9 in mouse brain is increased after transient focal cerebral ischemia
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DOI：
10.1097/00004647-200105000-00001
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发表时间：
2001-05-01
影响因子：
6.3
通讯作者：
Regli, L
中科院分区：
文献类型：
--
作者：
Badaut, J;Hirt, L;Regli, L
水通道蛋白9 （aquaporin -9, AQP9）是水通道蛋白家族的新成员，主要表达于肝脏和睾丸。具有对各种溶质，特别是乳酸盐也具有渗透性的特点。最近的数据显示AQP9存在于大鼠大脑的伸长细胞上。在本研究中，作者展示了AQP9在小鼠脑星形胶质细胞中的表达及其在脑缺血后的表达变化。的确，在控制鼠标。AQP9免疫标记存在于蛛网膜下腔和脑室边缘的星形细胞突起上。在白质、海马、下丘脑和侧隔的星形胶质细胞上也观察到标记。局灶性短暂缺血后，梗死周围星形胶质细胞免疫标记增加。这项AQP9在小鼠大脑中的分布研究提示了AQP9在中枢神经系统水稳态中的作用。此外，AQP9在缺血病变周围星形胶质细胞上的过表达表明，AQP9也可能在缺血后水肿的调节中发挥作用，并且鉴于其对单羧酸盐的渗透性，在乳酸从缺血病灶的清除中发挥作用。
Aquaporin-9 (AQP9) is a new member of the aquaporin family of water-selective channels mainly expressed in liver and testis. presenting the characteristic of also being permeable to various solutes, particularly lactate. Recent data have shown the presence of AQP9 on tanycytes in the rat brain. In the current study, the authors show the expression of AQP9 in astrocytes in the mouse brain and changes in its expression after cerebral ischemia. Indeed, in control mouse. the AQP9 immunolabeling is present on astrocytic processes bordering the subarachnoid space and ventricles. The labeling also is observed on astrocytes in the white matter, hippocampus, hypothalamus, and lateral septum. After focal transient ischemia, on increase of the immunolabeling is detected on astrocytes in periinfarct areas. This AQP9 distribution study in mouse brain suggests a role of AQP9 in water homeostasis in the central nervous system. Furthermore, the overexpression of AQP9 on astrocytes surrounding an ischemic lesion suggests that AQP9 may also play a role in the regulation of postischemia edema and, in view of its permeability to monocarboxylates, in the clearance of lactate from the ischemic focus.