Ovine Progressive Pneumonia Virus Capsid Antigen as Found in CD163-and CD172a-Positive Alveolar Macrophages of Persistently Infected Sheep
Ovine Progressive Pneumonia Virus Capsid Antigen as Found in CD163-and CD172a-Positive Alveolar Macrophages of Persistently Infected Sheep
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DOI：
10.1177/0300985809359605
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发表时间：
2010-05-01
影响因子：
2.4
通讯作者：
Knowles, D. P.
中科院分区：
文献类型：
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作者：
Herrmann-Hoesing, L. M.;Noh, S. M.;Knowles, D. P.
对于受 OPPV 影响的组织，包括肺、乳腺、腕关节滑膜和大脑脉络丛，尚未描述绵羊进行性肺炎病毒 (OPPV) 的原位检测和携带 OPPV 的细胞的表型鉴定。在这项研究中，作者首先使用 2 种抗 CAEV CA 单克隆抗体 5A1 和 10A1 以及 2 个基于酶的 IHC 系统，开发了一种基于单酶的自动免疫组织化学 (IHC) 分析，用于检测 OPPV 感染组织上的 OPPV 衣壳抗原 (CA)。在 10 只自然持续感染 OPPV 的母羊中，1 只母羊的腕滑膜细胞间区域、3 只母羊肺组织中类似肺泡巨噬细胞和肺间质巨噬细胞的细胞以及 1 只母羊的乳腺肺泡细胞中检测到了 OPPV CA。此外，基于双酶的自动化 IHC 分析显示，OPPV CA 主要在肺和乳腺的 CD172a 或 CD163 阳性肺泡巨噬细胞中检测到。抗炎 (CD163) 和下调 (CD172a) 类型的肺泡巨噬细胞含有 OPPV CA，这导致了这样一种可能性：在 OPPV 持续感染期间，宿主肺泡巨噬细胞可能有助于限制炎症，而 OPPV 持续存在，未被肺和乳腺中宿主适应性免疫反应检测到。
In situ detection of ovine progressive pneumonia virus (OPPV) and the phenotypic identification of the cells that harbor OPPV have not been described for the OPPV-affected tissues, which include lung, mammary gland, synovial membranes of the carpal joint, and choroid plexus of the brain. In this study, the authors first developed a single enzyme-based automated immunohistochemical (IHC) analysis for detection of OPPV capsid antigen (CA) on OPPV-affected tissues, using 2 anti-CAEV CA monoclonal antibodies, 5A1 and 10A1, and 2 enzyme-based IHC systems. Out of 10 naturally and persistently OPPV-infected ewes, OPPV CA was detected in intercellular regions of the carpal synovial membrane of 1 ewe, in cells resembling alveolar macrophages and pulmonary interstitial macrophages in lung tissue of 3 ewes, and in mammary alveolar cells of 1 ewe. Furthermore, dual enzyme-based automated IHC analyses revealed that OPPV CA was predominantly detected in CD172a- or CD163-positive alveolar macrophages of the lungs and mammary gland. That anti-inflammatory (CD163) and downregulatory (CD172a) types of alveolar macrophage harbor OPPV CA leads to the possibility that during persistent infection with OPPV, the host alveolar macrophage might serve to limit inflammation while OPPV persists undetected by the host adaptive immune response in the lung and mammary gland.