Changes in the T-helper cytokine profile and in lymphocyte activation at the systemic and local levels in women with endometriosis
Changes in the T-helper cytokine profile and in lymphocyte activation at the systemic and local levels in women with endometriosis
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DOI：
10.1016/j.fertnstert.2005.05.066
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发表时间：
2005-12-01
影响因子：
6.7
通讯作者：
Shor, AL
中科院分区：
文献类型：
--
作者：
Antsiferova, YS;Sotnikova, NY;Shor, AL
目的：评估子宫内膜异位症患者外周血和子宫内膜组织中调节性细胞因子的合成和淋巴细胞活化。设计：对照临床研究。地点：医疗中心。患者：15 名经腹腔镜诊断为子宫内膜异位症的女性； 20 名以前有生育记录的妇科健康女性（对照组）：干预措施：外周静脉血采样；主要结果指标（S）：通过实时逆转录酶聚合酶链反应评估外周和子宫内膜淋巴细胞中白细胞介素（IL）-2、IL-4和IL-10表达的信使RNA（mRNA）；通过流式细胞术建立这些细胞因子的细胞内合成和淋巴细胞表型谱。结果：外周淋巴细胞中IL-4和IL-10的mRNA表达和细胞内合成均急剧增加。在患有子宫内膜异位症的女性的异位子宫内膜中观察到 IL-4 的相同变化。同时，在子宫内膜异位病灶中观察到泛B细胞、CD20+CD5+B-1细胞和活化的HLA-DR+CD20+B淋巴细胞数量升高。在位子宫内膜中仅观察到 B 淋巴细胞数量增加。结论：子宫内膜异位症的发展伴随着全身和局部水平的 T 辅助细胞 2 型免疫反应的激活。我们的结果支持有关子宫内膜异位症自身免疫性质的假设，并可以解释子宫内膜异位症患者自身抗体产生的高水平。
Objective: To estimate regulatory cytokine synthesis and lymphocyte activation in the peripheral blood and I endometrial tissue of patients with endometriosis.Design: Controlled clinical study.Setting: Medical center.Patient(s): Fifteen women with laparoscopically diagnosed endometriosis; 20 gynecologically healthy women with previously documented fertility (control group):Intervention(s): Peripheral venous blood sampling; laparoscopic collection of ectopic and matched eutopic endometrium.Main Outcome Measure(S): Messenger RNA (mRNA) for interleukin (IL)-2, IL-4, and IL-10 expression in peripheral and endometrium lymphocytes was assessed by real-time reverse transcriptase polymerase chain reaction; intracellular synthesis of these cytokines and lymphocyte phenotype profile were established by flow cytometry.Result(s): Both mRNA expression and intracellular synthesis of IL-4 and IL-10 were sharply increased in peripheral lymphocytes. The same changes ere observed for IL-4 in ectopic endometrium of women with endometriosis. Simultaneously, elevation of the amount of pan-B cells, CD20+CD5+ B-1 cells, and activated HLA-DR+CD20+ B lymphocytes was observed in endometriosis lesions. Only an enhanced amount of B lymphocytes was seen in eutopic endometrium.Conclusion(s): Endometriosis development is accompanied by the activation of a T-helper type 2 immune response at the systemic and local levels. Our results support the hypothesis regarding the autoimmune nature of endometriosis and can explain the high level of autoantibody production in patients with endometriosis.