Mapping Synapses by Conjugate Light-Electron Array Tomography
Mapping Synapses by Conjugate Light-Electron Array Tomography
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DOI：
10.1523/jneurosci.4274-14.2015
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发表时间：
2015-04-08
影响因子：
5.3
通讯作者：
Smith, Stephen J.
中科院分区：
文献类型：
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作者：
Collman, Forrest;Buchanan, Joann;Smith, Stephen J.
哺乳动物中枢神经系统的突触在大小、结构、分子组成和功能上是不同的。突触在其无数的变化是神经回路的发展，稳态，可塑性和记忆存储的基础。不幸的是，定量分析和映射的大脑的异质性突触群体一直受到限制，缺乏足够的单突触测量方法。电子显微镜（EM）是识别和测量单个突触接触的决定性手段，但EM仅具有有限的能力来测量突触的分子组成。本报告介绍了共轭阵列断层扫描（AT），体积成像方法，集成了免疫荧光和EM成像方式的体素共轭的方式。我们说明了使用共轭AT推进EM验证的单突触分析在小鼠皮层的研究中的蛋白质测量。
Synapses of the mammalian CNS are diverse in size, structure, molecular composition, and function. Synapses in their myriad variations are fundamental to neural circuit development, homeostasis, plasticity, and memory storage. Unfortunately, quantitative analysis and mapping of the brain's heterogeneous synapse populations has been limited by the lack of adequate single-synapse measurement methods. Electron microscopy (EM) is the definitive means to recognize and measure individual synaptic contacts, but EM has only limited abilities to measure the molecular composition of synapses. This report describes conjugate array tomography (AT), a volumetric imaging method that integrates immunofluorescence and EM imaging modalities in voxel-conjugate fashion. We illustrate the use of conjugate AT to advance the proteometric measurement of EM-validated single-synapse analysis in a study of mouse cortex.