Application of eco-compatible biochar in anaerobic digestion to relieve acid stress and promote the selective colonization of functional microbes
Application of eco-compatible biochar in anaerobic digestion to relieve acid stress and promote the selective colonization of functional microbes
复制标题
生态相容性生物炭在厌氧消化中的应用缓解酸胁迫并促进功能微生物的选择性定殖
DOI：
10.1016/j.watres.2014.10.052
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发表时间：
2015-01-01
期刊：
影响因子：
12.8
通讯作者：
He, Pinjing
中科院分区：
文献类型：
--
作者：
Luo, Chenghao;Lu, Fan;He, Pinjing
将0.5-1 mm生物稳定的生物炭（10 g/L）添加到接种有破碎颗粒的中温厌氧消化器中（1g-VS/L）和4、6和8 g/L葡萄糖分别使产甲烷滞后期缩短11.4%、30.3%和21.6%，最大产甲烷速率提高86.6%、21.4%和5.2%。与不含生物炭的对照组相比。在葡萄糖负荷为6 g/L时，添加75 μ m生物炭使滞后期进一步缩短了38.0%，甲烷产率提高了70.6%。生物炭还同时增强了中间酸的产生和降解。指纹和测序分析用于检查的空间分布和时间演变的社区显示，比例较高的生物炭添加的治疗和紧密结合的馏分。甲烷八叠球菌主要分布在生物炭表面紧密结合的部分，且在直径为2-5 mm的生物炭颗粒中数量最多。甲烷菌富集在松散结合的馏分由所有大小的生物炭颗粒和紧密结合的馏分内的小生物炭颗粒。由于生物炭是具有成本效益的，可以保持在微生物状态，直接用作土壤改良剂，而无需分离，生态相容的生物炭可以作为一个很好的基质高负荷消化诱导功能微生物的选择性定植。(C)2014爱思唯尔有限公司版权所有。
The addition of 0.5-1 mm biostable biochar (10 g/L) to mesophilic anaerobic digesters inoculated with crushed granules (1 g-VS/L) and fed with 4, 6 and 8 g/L glucose shortened the methanogenic lag phase by 11.4%, 30.3% and 21.6% and raised the maximum methane production rate by 86.6%, 21.4% and 5.2%, respectively, compared with the controls without biochar. 75 mu m biochar further shortened the lag phase by 38.0% and increased the methane production rate by 70.6% at 6 g/L glucose loading. Biochar also simultaneously enhanced the production and degradation of intermediate acids. The fingerprint and sequencing analysis used to examine the spatial distribution and temporal evolution of communities revealed that proportion of Archaea was higher in the biochar-added treatments and in the tightly-bound fractions. Methanosarcina located in the tightly-bound fractions on the biochar surface, and was most abundant in the larger 2-5 mm biochar particles. Methanosaeta was enriched in the loosely-bound fractions by all-size biochar particles and within the tightly-bound fractions by small biochar particles. Because biochar is cost-effective and can remain in digestate for direct use as soil amendment without separation, eco-compatible biochar may serve as a good substrate for highly-loaded digestion by inducing selective colonization of functional microbes. (C) 2014 Elsevier Ltd. All rights reserved.