ENDONUCLEASE-III IS AN IRON SULFUR PROTEIN
ENDONUCLEASE-III IS AN IRON SULFUR PROTEIN
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DOI：
10.1021/bi00436a049
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发表时间：
1989-05-16
期刊：
影响因子：
2.9
通讯作者：
EMPTAGE, MH
中科院分区：
文献类型：
--
作者：
CUNNINGHAM, RP;ASAHARA, H;EMPTAGE, MH
元素分析、穆斯堡尔谱和EPR数据表明大肠杆菌的核酸内切酶III是一种铁硫蛋白。从大肠杆菌新鲜制备的蛋白质的穆斯堡尔谱。在富含57 Fe的培养基上生长的大肠杆菌证明天然酶含有2+氧化态的单个4Fe-4S簇，净自旋为零。在用derricyanide治疗后，一部分（
Elemental analyses, Mossbauer, and EPR data are reported to show that endonuclease III of Escherichia coli is an iron-sulfur protein. Mossbauer spectra of protein freshly prepared from E. coli grown on 57Fe-enriched medium demonstrate that the native enzyme contains a single 4Fe-4S cluster in the 2+ oxidation state, with a net spin of zero. Upon treatment with derricyanide, a fraction (