Diversity of fungal isolates from fungus‐growing termite Macrotermes barneyi and characterization of bioactive compound from Xylaria escharoidea
Diversity of fungal isolates from fungus‐growing termite Macrotermes barneyi and characterization of bioactive compound from Xylaria escharoidea
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DOI：
10.1111/1744-7917.12799
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发表时间：
2020-05
期刊：
影响因子：
4
通讯作者：
Venkateswarulu Nagam;Rammohan Aluru;M. Shoaib;G. Dong;Z. Li;Veera B. Pallaval;J. Ni
中科院分区：
文献类型：
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作者：
Venkateswarulu Nagam;Rammohan Aluru;M. Shoaib;G. Dong;Z. Li;Veera B. Pallaval;J. Ni
近年来，由于其潜在的应用和结构多样性，从昆虫相关真菌中发现新的次生代谢物引起了研究人员的兴趣。因此，本研究的目的是估计与真菌生长白蚁相关的真菌多样性，并对这些真菌进行潜在次生代谢产物的生物勘探。根据18S核糖体DNA基因序列分析，从巴尼大白蚁的肠道和梳子中分离到18种真菌。对所有已知真菌进行了抑菌活性测定，9株菌株对病原菌有活性。在实验室规模下培养了表现最好的木蝇，并对4,8‐二羟基‐3,4‐二氢萘- 1（2H）进行了分离和表征。分离得到的化合物对病原菌的最低抑制浓度为6.25 μg。此外，分子对接研究表明，4,8‐二羟基‐3,4‐二氢萘- 1（2H）是一种重要的抗菌剂，与调节辅助基因的蛋白a （agrAC）上的关键残基有明显的相互作用。本研究的发现支持了昆虫相关真菌抗菌特性的药物发现，这可能导致新的次级代谢物。
Owing to their potential applications, as well as their structural diversity, the discovery of novel secondary metabolites from insect‐associated fungi has been of interest to researchers in recent years. The aim of this study was therefore to estimate the diversity of fungi associated with fungus‐growing termites and bioprospecting these for potential secondary metabolites. In total, 18 fungal species were isolated and described from the gut and comb of Macrotermes barneyi based on 18S ribosomal DNA gene sequence analysis. Antimicrobial activity assays were carried out on all the known fungi, and nine isolates were recorded as active against pathogenic fungi. Xylaria escharoidea, the best performing isolate, was grown at laboratory scale and 4,8‐dihydroxy‐3,4‐dihydronaphthalen‐1(2H) was isolated and characterized. The minimum inhibitory concentration of this isolated compound against tested pathogenic organisms was found to be 6.25 μg. In addition, molecular docking studies have revealed that 4,8‐dihydroxy‐3,4‐dihydronaphthalen‐1(2H) is a prominent antibacterial agent with a marked interaction with key residues on protein A (agrAC) that regulates the accessory gene. The findings of this study support the drug discovery of antimicrobial properties in insect‐associated fungi, which may lead to novel secondary metabolites.