Ingestible Osmotic Pill for In Vivo Sampling of Gut Microbiomes
Ingestible Osmotic Pill for In Vivo Sampling of Gut Microbiomes
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DOI：
10.1002/aisy.201900053
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发表时间：
2019-09-01
影响因子：
7.4
通讯作者：
Sonkusale, Sameer R.
中科院分区：
文献类型：
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作者：
Nejad, Hojatollah Rezaei;Oliveira, Bruno C. M.;Sonkusale, Sameer R.
能够对结肠上游肠道不同部位进行无创采样的技术，使人们对器官特异性微生物群在人类健康中的作用有了新的认识。本文讨论了一种可消化的、生物相容的、无电池的、3d打印的微工程药丸，该药丸具有集成的渗透采样器和微流体通道，用于在体内采样肠腔及其结肠上游的微生物组。药丸的取样性能的特点是使用现实的体外模型和验证在猪和灵长类动物体内。在此，结果表明，从药丸的微流体通道中恢复的细菌种群与药丸所暴露的微环境的细菌种群统计数据非常相似。本文认为，这种药丸实验室装置彻底改变了对肠道微生物群空间多样性及其对医疗条件和治疗的反应的理解。
Technologies capable of noninvasively sampling different locations in the gut upstream of the colon enable new insights into the role of organ-specific microbiota in human health. Herein, an ingestible, biocompatible, battery-less, 3D-printed microengineered pill with an integrated osmotic sampler and microfluidic channels for in vivo sampling of the gut lumen and its microbiome upstream of the colon is discussed. The pill's sampling performance is characterized using realistic in vitro models and validated in vivo in pigs and primates. Herein, the results show that the bacterial populations recovered from the pill's microfluidic channels closely resemble the bacterial population demographics of the microenvironment to which the pill is exposed. Herein, it is believed that such lab-on-a-pill devices revolutionize the understanding of the spatial diversity of the gut microbiome and its response to medical conditions and treatments.