Anti-platelet effects of flavonoids and flavonoid-glycosides from Sophora japonica
Anti-platelet effects of flavonoids and flavonoid-glycosides from Sophora japonica
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DOI：
10.1007/s12272-001-1242-1
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发表时间：
2008-07-01
影响因子：
6.7
通讯作者：
Yun-Choi, Hye Sook
中科院分区：
文献类型：
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作者：
Kim, Jeong Mi;Yun-Choi, Hye Sook
对国槐的甲醇提取物进行抗血小板活性引导分馏，分离出四种黄酮类化合物和六种黄酮类苷：生物鸡丁 A (1)、伊立索酮 (2)、染料木黄酮 (3)、西索甙 (4)、槐二糖苷 (5)、染料木甙 (6)、鸢尾苷 (7)、芹菜素 (8)、槲皮甙(9)和芦丁(10)。通过多种光谱方法确定了每种化合物的结构。在这些化合物中，1、3和7对花生四烯酸(AA)和U46619诱导的血小板聚集的抑制作用比乙酰水杨酸(ASA，IC50：分别为19.9和99.8μM；20.3和53.8μM；25.9和123.4μM)相似，高2.5-6.5倍。 63.0和350.0μM）。化合物2对于AA和U46619诱导的聚集是类似于比ASA强22-40倍的抑制剂（IC50：分别为1.6和15.6μM）。
A methanol extract of Sophora japonica was subjected to anti-platelet activity guided fractionation affording the isolation of four flavonoids and six flavonoid-glycosides: biochanin A (1), irisolidone (2), genistein (3), sissotrin (4), sophorabioside (5), genistin (6), tectoridin (7), apigenin (8), quercitrin (9), and rutin (10). The structure of each compound was determined by a variety of spectroscopic methods. Among the compounds, 1, 3, and 7 showed similar to 2.5-6.5 fold greater inhibitory effects on arachidonic acid (AA) and U46619 induced platelet aggregation (IC50: 19.9 and 99.8 mu M; 20.3 and 53.8 mu M; 25.9 and 123.4 mu M, respectively) than acetylsalicylic acid (ASA, IC50: 63.0 and 350.0 mu M). Compound 2 was an similar to 22-40 fold stronger inhibitor than ASA on AA and U46619 induced aggregation (IC50: 1.6 and 15.6 mu M, respectively).