SIMPLE METHOD FOR MEASURING THE ANTIBIOTIC CONCENTRATION REQUIRED TO KILL ADHERENT BACTERIA
SIMPLE METHOD FOR MEASURING THE ANTIBIOTIC CONCENTRATION REQUIRED TO KILL ADHERENT BACTERIA
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DOI：
10.1159/000239015
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发表时间：
1992-09-01
期刊：
影响因子：
3.3
通讯作者：
SUGINAKA, H
中科院分区：
文献类型：
--
作者：
MIYAKE, Y;FUJIWARA, S;SUGINAKA, H
建立了一种测定塑料表面附着细菌对抗生素敏感性的简便方法。将附着在96孔塑料组织培养板底部的金黄色葡萄球菌细胞培养在连续稀释的抗生素溶液中。培养24小时后，取出溶液，每孔加入不含抗生素的新鲜培养液。通过进一步孵育24小时后细胞的生长来判断细胞的活性。杀死附着细菌所需的抗生素浓度远远高于浮游细胞所需的抗生素浓度，尽管我们使用了杀菌药物；β-内酰胺类、喹诺酮类和氨基糖苷类抗生素。黏附细胞表现出的耐受性可能在生物被膜感染的化疗中遇到的困难中发挥作用。
A simple method was developed for measuring the antibiotic susceptibility of bacteria adherent to plastic surfaces. Staphylococcus aureus cells adhered to the bottom of a 96-well plastic tissue culture plate were incubated in serially diluted antibiotic solutions. After a 24-hour-incubation the solutions were removed, and fresh medium without antibiotics was added to each well. The viability of the cells was judged by their growth after a further 24-hour-incubation. The antibiotic concentration required to kill adherent bacteria was far higher than that required for planktonic cells, although we used bactericidal drugs; beta-lactam, quinolone, and aminoglycoside antibiotics. The tolerance demonstrated by adherent cells is likely to play a role in the difficulties encountered in the chemotherapy of biofilm infections.