A morphological and electrophysiological study on the postnatal development of oligodendrocyte precursor cells in the rat brain
A morphological and electrophysiological study on the postnatal development of oligodendrocyte precursor cells in the rat brain
复制标题
大鼠脑内少突胶质前体细胞出生后发育的形态学和电生理学研究
DOI：
10.1016/j.brainres.2008.09.029
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发表时间：
2008-12-03
期刊：
影响因子：
2.9
通讯作者：
Deng, Qi-yue
中科院分区：
文献类型：
--
作者：
Chen, Peng-Hui;Cai, Wen-qin;Deng, Qi-yue
在CNS中广泛分布的细胞群通过特异性表达NG 2硫酸软骨素蛋白聚糖而被鉴定，并被命名为少突胶质细胞前体细胞（OPC）。OPCs可能具有干细胞样特征，包括体外和体内的多潜能性。有人提出CNS实质中的OPC包含独特的神经胶质细胞群，不同于少突胶质细胞和星形胶质细胞。本研究证实NG 2免疫反应阳性OPCs在生后不同发育阶段持续分布于大脑皮层和海马。这些细胞在出生后7天内数量迅速增加，并广泛迁移，在发育中的皮质和海马中充满丰富的突起。OPCs的形态变化极为复杂，从新生到成年，长距离初级突起的分布逐渐增多。免疫组化结果显示OPCs具有与星形胶质细胞相区别的形态学特征。OPCs的电生理特性表明，OPCs表达少量的内向Na+电流，这与神经元的Na+电流不同，因为OPCs具有较低的Na/K电导比和较高的指令电压阶跃去极化最大Na+电流幅度。这些观察结果表明，OPCs可以被鉴定为第三类大胶质细胞，因为它们在CNS中的分布，形态发育过程的多样性以及与星形胶质细胞的电生理差异。(C)2008 Elsevier B. V.保留所有权利。
A widespread population of cells in CNS is identified by specific expression of the NG2 chondroitin sulphate proteoglycan and named as oligodendrocyte precursor cell (OPC). OPCs may possess stem cell-like characteristics, including multipotentiality in vitro and in vivo. it was proposed that OPCs in the CNS parenchyma comprise a unique population of glia, distinct from oligodendrocytes and astrocytes. This study confirmed that NG2 immunoreactive OPCs were continuously distributed in cerebral cortex and hippocampus during different postnatal developmental stages. These cells rapidly increased in number over the postnatal 7 days and migrate extensively to populate with abundant processes both in developing cortex and hippocampus. The morphology of OPCs exhibited extremely complex changes with the distribution of long distance primary process gradually increased from neonatal to adult CNS. Immunohistochemical studies showed that OPCs exhibited the morphological properties that can be distinguished from astrocytes. The electrophysiological properties showed that OPCs expressed a small amount of inward Na+ currents which was distinguished from Na+ currents in neurons owing to their lower Na-to-K conductance ratio and higher command voltage step depolarized maximum Na+ current amplitude. These observations suggest that OPCs can be identified as the third type of macroglia because of their distribution in the CNS, the morphological development in process diversity and the electrophysiological difference from astrocyte. (C) 2008 Elsevier B.V. All rights reserved.