Meiotic H3K9me2 distribution is influenced by the ALG-3 and ALG-4 pathway and by poly(U) polymerase activity.
Meiotic H3K9me2 distribution is influenced by the ALG-3 and ALG-4 pathway and by poly(U) polymerase activity.
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DOI：
10.17912/micropub.biology.000455
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发表时间：
2021
影响因子：
--
通讯作者：
Maine EM
中科院分区：
文献类型：
--
作者：
Li Y;Snyder M;Maine EM
组蛋白修饰通过改变染色质结构和非组蛋白的募集来影响基因表达和染色体动力学。组蛋白H3在赖氨酸9上的二甲基化（H3 K9 me 2）是常在异染色质内发现的保守修饰。在第一次减数分裂前期同源染色体进行配对和联会时，免疫标记C。线虫雄性生殖细胞在单个X染色体上检测到相对高的H3 K9 me 2水平，而在突触常染色体上检测到相对低的H3 K9 me 2水平。这种H3 K9 me 2分布受小RNA机器的几种组分的影响，包括：EGO-1 RNA指导的RNA聚合酶（RdRP）; DRH-3解旋酶; EKL-1，Tudor结构域蛋白; CSR-1 Argonaute;和RRF-3 RdRP。EG 0 -1、DRH-3和EKL-1共同起作用以在生殖系中产生/稳定22 G RNA。这些22 G RNA的一个子集与CSR-1一起发挥作用，以确保正确的基因表达。RRF-3 RdRP在26 G RNA的生物发生中起作用，26 G RNA进入由ERGO-1 Argonaute和冗余ALG-3和ALG-4 Argonaute蛋白介导的两种种系调节机制。在这里，我们报告说，减数分裂H3 K9 me 2分布的ALG-3和ALG-4，以及所需的26 G RNA合成，ERI-1和ERI-5的其他两个因素的影响。此外，减数分裂H3 K9 me 2分布受聚（U）聚合酶PUP-1（又名CDE-1，CID-1）和PUP-2的活性影响。
Histone modifications influence gene expression and chromosome dynamics by altering chromatin structure and recruitment of nonhistone proteins. Dimethylation of histone H3 on lysine 9 (H3K9me2) is a conserved modification often found within heterochromatin. During first meiotic prophase when homologous chromosomes undergo pairing and synapsis, immunolabeling of C. elegans male germ cells detects a relatively high H3K9me2 level on the single X chromosome and a relatively low H3K9me2 level on synapsed autosomes. This H3K9me2 distribution is influenced by several components of the small RNA machinery, including: EGO-1 RNA-directed RNA polymerase (RdRP); DRH-3 helicase; EKL-1, a Tudor domain protein; CSR-1 Argonaute; and RRF-3 RdRP. EGO-1, DRH-3, and EKL-1 function together to generate/stabilize 22G RNAs in the germ line. A subset of these 22G RNAs function together with CSR-1 to ensure correct gene expression. RRF-3 RdRP functions in biogenesis of 26G RNAs that feed into two germline regulatory mechanisms mediated by ERGO-1 Argonaute and the redundant ALG-3 and ALG-4 Argonaute proteins. Here, we report that meiotic H3K9me2 distribution is influenced by ALG-3 and ALG-4, as well as by two other factors required for 26G RNA synthesis, ERI-1 and ERI-5. Moreover, meiotic H3K9me2 distribution is influenced by activity of the poly(U) polymerases, PUP-1 (aka CDE-1, CID-1) and PUP-2.