Antioxidant and immunological activities of water-soluble polysaccharides from Aconitum kusnezoffii Reichb.
Antioxidant and immunological activities of water-soluble polysaccharides from Aconitum kusnezoffii Reichb.
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DOI：
10.1016/j.ijbiomac.2011.06.017
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发表时间：
2011-11-01
影响因子：
8.2
通讯作者：
Tao, Yanduo
中科院分区：
文献类型：
--
作者：
Gao, Tingting;Ma, Shuai;Tao, Yanduo
乌头草。乌头是记载最早的有毒植物之一，在过去的2500年里，乌头一直被用作中药和药疗，治疗心力衰竭、充血、神经痛、风湿病和痛风等。本研究从冬青块茎中分离得到4个水溶性多糖组分。首次对其抗氧化和免疫活性进行了研究。体外抗氧化实验表明，WKCP-A组分对DPPH自由基、羟基自由基、超氧阴离子、H2O2具有明显的清除活性，对1,2,3-苯三醇的自氧化、亚铁离子的螯合能力和还原能力。此外，体内免疫实验表明，WKCP-A和WKHP组分对脾淋巴细胞增殖和巨噬细胞吞噬的促进作用比其他组分更显著。因此，对草麻多糖的水溶性进行了初步研究。特别是WKCP-A，具有开发新型天然抗氧化剂和免疫刺激剂的潜力，可用于功能性食品或药物。(C) 2011 Elsevier B.V.版权所有
Aconitum kusnezoffii Reichb., one of the earliest recorded toxic species of genus Aconitum, has been used as traditional Chinese medicine and medicinal diet over the last 2500 years to treat heart failure congestion, neuralgia, rheumatism and gout, etc. In the present paper, four water-soluble polysaccharide fractions isolated from the tubers of A. kusnezoffii Reichb. were studied the antioxidant and immunological activities for the first time. In vitro antioxidant assays indicated that fraction WKCP-A had noticeable scavenging activities on DPPH radical, hydroxyl radical, superoxide anion, H2O2 and self-oxidation of 1,2,3-phentriol, ferrous ion-chelating ability and reducing power. Moreover, the in vivo immunological assay exhibited that fractions WKCP-A and WKHP could more significantly enhance splenic lymphocyte proliferation and macrophage phagocytosis than other fractions. Therefore, the water-soluble polysaccharides from A. kusnezoffii Reichb., especially WKCP-A, have the potential to be explored as novel natural antioxidants and immunostimulating agents for using in functional foods or medicine. (C) 2011 Elsevier B.V. All rights reserved.