Differential APC requirements of self- and nonself-reactive T cells and T cell hybridomas specific for retinal S-antigen.
Differential APC requirements of self- and nonself-reactive T cells and T cell hybridomas specific for retinal S-antigen.
复制标题
自身和非自身反应性 T 细胞以及视网膜 S 抗原特异性 T 细胞杂交瘤的 APC 需求差异。
DOI：
10.1006/jaut.1996.0112
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Gregerson,DS
中科院分区：
文献类型：
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作者：
Prasad,SA;Fling,SP;Gregerson,DS
视网膜S-Ag的致病性肽特异性自身反应性T细胞杂交瘤需要一种新的放射敏感性APC活性IL-2分泌，这与MHC II类的Ag呈递不同。抗原依赖性的IL-2分泌的自身反应性杂交瘤是更有效的脾APC比胸腺APC，虽然两者提供了类似的水平的杂交瘤TCR占用，通过激活诱导的细胞死亡。此外，胸腺APC刺激非自身反应性杂交瘤分泌IL-2。为了检验这种活性由不同的细胞群提供的假设，检测了分级分离的脾细胞向这些杂交瘤呈递Ag的能力。发现用于IL-2分泌的最有效的Ag呈递与低密度、B细胞富集的组分分离，而粘附细胞或纯化的T细胞不能支持IL-2产生。与以前的结果一起，数据显示，导致这些自身反应性T细胞杂交瘤分泌IL-2的抗原呈递需要活化的B细胞，而TCR占用可以由几个APC亚群提供。
Autoreactive T cell hybridomas specific for pathogenic peptides of retinal S-Ag require a novel radiosensitive APC activity for IL-2 secretion that is distinct from Ag presentation by MHC class II. Antigen-dependent IL-2 secretion by self-reactive hybridomas was much more efficient with splenic APC than with thymic APC, although both provided similar levels of hybridoma TCR occupancy as measured by activation-induced cell death. Furthermore, thymic APC did stimulate IL-2 secretion by a non-self reactive hybridoma. To test the hypothesis that this activity was provided by a distinct cell population, fractionated splenocytes were tested for their ability to present Ag to these hybridomas. The most potent Ag presentation for IL-2 secretion was found to segregate with low-density, B cell-enriched fractions while adherent cells, or purified T cells were unable to support IL-2 production. Together with previous results, the data show that antigen presentation leading to IL-2 secretion by these autoreactive T cell hybridomas requires activated B cells, whereas TCR occupancy can be provided by several APC subsets.