Non-invasive monitoring of skin inflammation using an oxygen-sensing paint-on bandage.
Non-invasive monitoring of skin inflammation using an oxygen-sensing paint-on bandage.
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DOI：
10.1364/boe.8.004640
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发表时间：
2017-10
影响因子：
3.4
通讯作者：
Zongxi Li;N. Navarro-Alvarez;E. J. Keeley;Nicholas H. Nowell;Beatriz M M Gonçalves-Beatriz-M-M-Gonçalves-39693886;Christene A. Huang;C. Evans
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文献类型：
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作者：
Zongxi Li;N. Navarro-Alvarez;E. J. Keeley;Nicholas H. Nowell;Beatriz M M Gonçalves-Beatriz-M-M-Gonçalves-39693886;Christene A. Huang;C. Evans
炎症涉及一系列细胞和分子介质，最终导致免疫细胞渗透到受影响的区域。皮肤中的这种炎症过程在许多疾病中都很常见，包括痤疮、感染和牛皮癣，免疫细胞的存在或不存在是潜在的诊断标志。在这里，我们展示了在活体猪炎症模型中，使用涂抹的氧气感应绷带可以非侵入性地测量和映射皮肤炎症。在注射已知的炎症剂后，绷带可以跟踪损伤部位超过7周的氧耗量的增加、平台和下降，以及识别皮肤表面下不同深度注射所产生的炎症。PO2的初始变化速率和平衡时绷带PO2的变化(CBP20)都与接触组织的代谢耗氧速率直接相关。健康皮肤的初始PO2下降率为6.5[公式：见正文]，CBP20为84[公式：见正文]。发炎皮肤的初始消耗率显著更高，为55[配方：见文本]，更大的CBP20为140[配方：见文本]。研究发现，绷带在与组织平衡前后的PO2变化与动物皮肤炎症的组织学证据有很好的相关性。
Inflammation involves a cascade of cellular and molecular mediators that ultimately lead to the infiltration of immune cells into the affected area. This inflammatory process in skin is common to many diseases including acne, infection, and psoriasis, with the presence or absence of immune cells a potential diagnostic marker. Here we show that skin inflammation can be non-invasively measured and mapped using a paint-on oxygen sensing bandage in an in vivo porcine inflammation model. After injection of a known inflammatory agent, the bandage could track the increase, plateau, and decrease in oxygen consumption at the injury site over 7 weeks, as well as discern inflammation resultant from injection at various depths beneath the surface of the skin. Both the initial rate of pO2 change and the change in bandage pO2 at equilibration (CBP20) were found to be directly related to the metabolic oxygen consumption rate of the tissue in contact. Healthy skin demonstrated an initial pO2 decrease rate of 6.5 [Formula: see text], and CBP20 of 84 [Formula: see text]. Inflamed skin had a significantly higher initial consumption rate of 55 [Formula: see text], and a larger CBP20 of 140 [Formula: see text]. The change in the bandage pO2 before and after equilibration with tissue was found to correlate well with histological evidence of skin inflammation in the animals.