RETRACTED ARTICLE: Characterization of cancer stem-like cells in the side population cells of human gastric cancer cell line MKN-45
RETRACTED ARTICLE: Characterization of cancer stem-like cells in the side population cells of human gastric cancer cell line MKN-45
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DOI：
10.1631/jzus.b1200102
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发表时间：
2013-03
期刊：
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通讯作者：
Hai-hong Zhang;A. Cai;Xue-ming Wei;L. Ding;Feng-zhi Li;Ai-ming Zheng;D. Dai;Rong-rong Huang;Hou-jun Cao;Haiyun Zhou;Jian-mei Wang;Xue-jing Wang;Wei Shi;Heng Zhu;Xiao-ying Yuan;Lin Chen
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作者：
Hai-hong Zhang;A. Cai;Xue-ming Wei;L. Ding;Feng-zhi Li;Ai-ming Zheng;D. Dai;Rong-rong Huang;Hou-jun Cao;Haiyun Zhou;Jian-mei Wang;Xue-jing Wang;Wei Shi;Heng Zhu;Xiao-ying Yuan;Lin Chen
侧群（SP）细胞可能在肿瘤发生和癌症复发中起关键作用。SP细胞在多种细胞系和组织中均有表达，包括多种胃癌细胞系。本研究旨在鉴定胃癌细胞系MKN-45 SP中的癌干细胞样细胞。我们使用荧光激活细胞分选（FACS）分选SP细胞在人胃癌细胞系MKN-45（细胞标记Hoechst 33342），然后表征SP细胞的癌干细胞样特性。本研究发现SP细胞比MP细胞具有更高的克隆形成效率。采用实时定量逆转录聚合酶链反应（RT-PCR）检测SP和MP细胞中与干细胞相关的OCT-4、SOX-2、NANOG、CD 44和三磷酸腺苷（ATP）结合盒转运体基因ABCG 2的表达谱。Western blot检测SP和MP细胞蛋白表达的差异。这两个结果表明，SP细胞中的蛋白表达显著高于MP细胞。接种非肥胖型糖尿病/严重联合免疫缺陷（NOD/SCID）小鼠后，SP细胞比MP细胞具有更高的致瘤倾向。这些结果表明SP细胞具有癌干细胞特性，并证明来自MKN-45的SP细胞是胃癌干细胞样细胞。
Side population (SP) cells may play a crucial role in tumorigenesis and the recurrence of cancer. Many kinds of cell lines and tissues have demonstrated the presence of SP cells, including several gastric cancer cell lines. This study is aimed to identify the cancer stem-like cells in the SP of gastric cancer cell line MKN-45. We used fluorescence activated cell sorting (FACS) to sort SP cells in the human gastric carcinoma cell line MKN-45 (cells labeled with Hoechst 33342) and then characterized the cancer stem-like properties of SP cells. This study found that the SP cells had higher clone formation efficiency than major population (MP) cells. Five stemness-related gene expression profiles, including OCT-4, SOX-2, NANOG, CD44, and adenosine triphosphate (ATP)-binding cassette transporters gene ABCG2, were tested in SP and MP cells using quantitative real-time reverse transcription polymerase chain reaction (RT-PCR). Western blot was used to show the difference of protein expression between SP and MP cells. Both results show that there was significantly higher protein expression in SP cells than in MP cells. When inoculated into non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice, SP cells show higher tumorigenesis tendency than MP cells. These results indicate that SP cells possess cancer stem cell properties and prove that SP cells from MKN-45 are gastric cancer stem-like cells.