Protein subunit vaccines of parainfluenza type 3 virus: immunogenic effect in lambs and mice.
Protein subunit vaccines of parainfluenza type 3 virus: immunogenic effect in lambs and mice.
复制标题
3型副流感病毒蛋白亚单位疫苗：对羔羊和小鼠的免疫原性作用。
DOI：
10.1099/0022-1317-64-7-1557
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
K. Simons
中科院分区：
文献类型：
--
作者：
B. Morein;M. Sharp;B. Sundquist;K. Simons
蛋白亚单位疫苗由副流感病毒3型（PI-3）的血凝素（HN）和融合（F）糖蛋白的混合物制备。糖蛋白以三种不同的形式分离，并通过其沉降系数进行表征：30 S蛋白胶束（几种HN和F糖蛋白的复合物，不含去污剂和脂质），18 S蛋白-TX复合物（几种糖蛋白的复合物，含有去污剂Triton X-100）和4S蛋白-TX复合物（可能是与Triton X-100复合的糖蛋白单体）。这些制剂作为疫苗在小鼠和羔羊中进行了试验。使用ELISA技术在血清和肺提取物中测定小鼠的免疫应答。两种多聚体复合物都具有高度免疫原性。30 S蛋白胶束在两次注射10或1微克蛋白后诱导高抗体应答。血清IgG滴度分别达到约90 μ g/ml和40 μ g/ml的水平。用18 S蛋白-TX复合物达到相似的滴度。两次注射30 S或18 S复合物后，在肺提取物中检测到伊加抗体应答。4S蛋白-TX复合物是弱免疫原，在小鼠中诱导低抗体应答。羔羊接种30 S蛋白胶团，并通过血清学和攻毒实验评估免疫反应。油佐剂中的30 S蛋白胶束诱导可检测的血清抗体滴度以及针对由PI-3病毒引起的肺炎的保护性免疫。
Protein subunit vaccines were prepared from a mixture of the haemagglutinin (HN) and fusion (F) glycoproteins of parainfluenza type 3 virus (PI-3). The glycoproteins were isolated in three different forms and characterized by their sedimentation coefficients: 30S protein micelles (a complex of several HN and F glycoproteins devoid of detergent and lipid), 18S protein-TX complexes (a complex of several glycoproteins containing the detergent Triton X-100), and 4S protein-TX complexes (probably monomers of the glycoproteins complexed to Triton X-100). These preparations were tested as vaccines in mice and lambs. The immune response in the mice was assayed both in the serum and in extracts from the lungs using an ELISA technique. Both of the multimeric complexes were highly immunogenic. The 30S protein micelles induced a high antibody response after two injections with either 10 or 1 microgram protein. The serum IgG titres reached levels of about 90 micrograms/ml and 40 micrograms/ml respectively. Similar titres were reached with the 18S protein-TX complexes. After two injections of either the 30S or the 18S complexes IgA antibody responses were detected in the lung extracts. The 4S protein-TX complexes were poor immunogens and induced low antibody responses in mice. The lambs were vaccinated with the 30S protein micelles, and the immune response was evaluated serologically and in challenge experiments. The 30S protein micelles in an oil adjuvant induced detectable serum antibody titres as well as protective immunity against the pneumonia caused by the PI-3 virus.
单纯疱疹疫苗。
DOI：
10.1016/b978-0-7506-9265-6.50016-6
发表时间：
1992
期刊：
Biotechnology (Reading, Mass.)
影响因子：
--
作者：
Whitley,RJ;Meignier,B
通讯作者：
Meignier,B