ENDOTHELIAL TYPE NITRIC-OXIDE SYNTHASE IN SKELETAL-MUSCLE FIBERS - MITOCHONDRIAL RELATIONSHIPS
ENDOTHELIAL TYPE NITRIC-OXIDE SYNTHASE IN SKELETAL-MUSCLE FIBERS - MITOCHONDRIAL RELATIONSHIPS
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DOI：
10.1006/bbrc.1995.1824
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发表时间：
1995-06-15
影响因子：
3.1
通讯作者：
STAMLER, JS
中科院分区：
文献类型：
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作者：
KOBZIK, L;STRINGER, B;STAMLER, JS
免疫组化显示，骨骼肌纤维表达内皮型（ec）-NOS的异质模式不同的神经元（NC）-NOS。相邻连续切片的分析揭示：1）ec-NOS表达与线粒体含量的强相关性（通过琥珀酸脱氢酶的组织化学观察）; 2）ec-NOS与纤维ATP酶类型缺乏相关性（与nc-NOS相反）;以及3）在一些纤维中组成性共表达ec-和nc-NOS。从膈肌制备的线粒体表现出钙依赖性NOS活性，其功能是抑制体外耗氧。结果表明，正常骨骼肌细胞可以表达两种组成型的NOS（NC-和EC-），并建议在氧化的，富含纤维的EC-NOS的功能作用。(C)出版社：Academic Press
Immunohistochemistry shows that skeletal muscle fibers express endothelial-type (ec)-NOS in a heterogeneous pattern distinct from that of neuronal (nc)-NOS. Analysis of adjacent serial sections reveals: 1) a strong correlation of ec-NOS expression to mitochondrial content (visualized histochemically by succinate dehydrogenase); 2) lack of correlation of ec-NOS to fiber ATPase type (in contrast to nc-NOS); and 3) constitutive co-expression of ec- and nc-NOS in some fibers. Preparations of mitochondria from diaphragm exhibited calcium-dependent NOS activity, which functioned to inhibit in vitro oxygen consumption. The results establish that normal skeletal muscle cells can express two constitutive isoforms of NOS (nc- and ec-) and suggest a functional role for ec-NOS in oxidative, mitochondria-rich fibers. (C) 1995 Academic Press, Inc.