Tissue Engineered Vascular Graft Recipient Interleukin 10 Status Is Critical for Preventing Thrombosis.
Tissue Engineered Vascular Graft Recipient Interleukin 10 Status Is Critical for Preventing Thrombosis.
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DOI：
10.1002/adhm.202001094
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发表时间：
2020-12
影响因子：
10
通讯作者：
Breuer CK
中科院分区：
文献类型：
--
作者：
Mirhaidari GJM;Barker JC;Zbinden JC;Santantonio BM;Chang YC;Best CA;Reinhardt JW;Blum KM;Yi T;Breuer CK
组织工程血管移植（TEVGs）是一项很有前途的技术，但受到闭塞的阻碍。用骨髓来源的单核细胞（BM-MNCs）播种可减轻闭塞，但确切的机制尚不清楚。种子细胞迅速消失，并可能通过旁分泌信号介导抗炎作用。在这里，我们报道了一系列互惠的遗传TEVG植入和重组蛋白处理，以研究宿主和种子细胞中白细胞介素-10（一种抗炎细胞因子）的作用。将野生型和IL-10小鼠的BM-MNCs植入tevg，再加上未播种的移植物，植入野生型和IL-10小鼠。未经种子移植的野生型小鼠也接受重组IL-10。连续超声评估闭塞性，14天收集tevg进行免疫组织化学分析。由于急性（<3天）血栓形成，IL-10 KO小鼠的TEVGs闭塞发生率明显高于野生型小鼠。细胞播种在IL-10 KO小鼠中拯救tevg，与野生型开放相当。来自宿主和种子细胞的IL-10对移植物炎症和巨噬细胞表型没有显著影响，但IL-10处理显示出有趣的生物学效应，包括降低细胞增殖和增加M2巨噬细胞极化。来自宿主的IL-10对预防TEVG血栓形成至关重要，种子BM-MNCs在IL-10 KO小鼠中具有显著的抗血栓作用。
Tissue engineered vascular grafts (TEVGs) are a promising technology, but are hindered by occlusion. Seeding with bone-marrow derived mononuclear cells (BM-MNCs) mitigates occlusion, yet the precise mechanism remains unclear. Seeded cells disappear quickly and potentially mediate an anti-inflammatory effect through paracrine signaling. Here, we report a series of reciprocal genetic TEVG implantations plus recombinant protein treatment to investigate what role interleukin-10, an anti-inflammatory cytokine, plays from both host and seeded cells. TEVGs seeded with BM-MNCs from wild-type and IL-10 KO mice, plus unseeded grafts, were implanted into wild-type and IL-10 KO mice. Wild-type mice with unseeded grafts also received recombinant IL-10. Serial ultrasound evaluated occlusion and TEVGs were harvested at 14 days for immunohistochemical analysis. TEVGs in IL-10 KO mice had significantly higher occlusion incidence compared to wild-type mice attributed to acute (<3day) thrombosis. Cell seeding rescued TEVGs in IL-10 KO mice comparable to wild-type patency. IL-10 from the host and seeded cells did not significantly influence graft inflammation and macrophage phenotype, yet IL-10 treatment showed interesting biologic effects including decreasing cell proliferation and increasing M2 macrophage polarization. IL-10 from the host is critical for preventing TEVG thrombosis and seeded BM-MNCs exert a significant anti-thrombotic effect in IL-10 KO mice.
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DOI：
10.1016/j.jtcvs.2009.09.057
发表时间：
2010-02-01
影响因子：
6
作者：
Hibino, Narutoshi;McGillicuddy, Edward;Shinoka, Toshiharu
通讯作者：
Shinoka, Toshiharu
影响因子：
3
作者：
Melchiorri, Anthony J.;Bracaglia, Laura G.;Fisher, John P.
通讯作者：
Fisher, John P.
影响因子：
4.8
作者：
Hibino, Narutoshi;Mejias, Dane;Breuer, Christopher
通讯作者：
Breuer, Christopher
影响因子：
6
作者：
Eming, Sabine A.;Werner, Sabine;Roers, Axel
通讯作者：
Roers, Axel
影响因子：
3.5
作者：
Miyachi, Hideki;Reinhardt, James W.;Shinoka, Toshiharu
通讯作者：
Shinoka, Toshiharu