Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment
Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment
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对仪器平台和实验工作流程进行实验室间评估，以进行定量准确性和再现性评估
DOI：
10.1016/j.euprot.2015.06.001
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发表时间：
2015
影响因子：
--
通讯作者：
C. Borchers
中科院分区：
文献类型：
--
作者：
A. Percy;Jessica Tamura;J. Albar;K. Aloria;A. Amirkhani;Gabriel D. T. Araujo;J. M. Arizmendi;F. Blanco;F. Canals;Jin‐Young Cho;Núria Colomé;F. Corrales;G. Domont;Guadalupe Espadas;P. Fernández‐Puente;C. Gil;P. Haynes;M. Hernáez;Jin;A. Kopylov;Miguel Marcilla;Mathew J. McKay;M. Mirzaei;M. Molloy;Leanne B. Ohlund;Y. Paik;A. Paradela;M. Raftery;E. Sabidó;L. Sleno;Daniel Wilffert;J. Wolters;J. Yoo;V. Zgoda;C. Parker;C. Borchers
在一项涉及 16 个实验室和 19 个 LC-MS/MS 平台的大规模国际研究中，使用两种旨在评估仪器性能的试剂盒和一种旨在评估整个自下而上工作流程的试剂盒，检查了实验室之间和仪器类型之间血浆蛋白定量的重现性。仪器类型对结果质量影响很小，这证明了 LC/MRM-MS 与同位素标记标准品的稳健性。技术人员的技能是一个因素，因为样品制备中的错误和次优的 LC-MS 性能是显而易见的。这凸显了在对患者样本进行定量之前进行适当培训和常规质量控制的重要性。
The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC–MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC–MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples.