Free radical-scavenging activities of Crataegus monogyna extracts
Free radical-scavenging activities of Crataegus monogyna extracts
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DOI：
10.3390/medicina44090091
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发表时间：
2008-01-01
影响因子：
2.6
通讯作者：
Peciura, Rimantas
中科院分区：
文献类型：
--
作者：
Bernatoniene, Jurga;Masteikova, Ruta;Peciura, Rimantas
本研究的目的是研究山楂果实水提取物和乙醇提取物、其类黄酮和类黄酮组合的抗自由基活性。材料和方法。使用高效液相色谱法测定酚类化合物的总量和黄酮类化合物的成分。采用分光光度法（二苯基-1-三硝基苯肼（DPPH 中心点）自由基清除法和 2,2'-连氮基双酶法）定量测定山楂提取物和黄酮类化合物（绿原酸、金丝桃苷、芦丁、槲皮素、牡荆素-2O-鼠李糖苷、表儿茶素、儿茶素和原花青素 B-2）的抗氧化活性。 (3-乙基苯并噻唑啉-6-磺酸)(ABTS中心点+)自由基阳离子脱色测定)。使用高效液相色谱法测定酪氨酸硝化抑制水平。结果。山楂果实乙醇提取物含有 182 +/- 4 mg/100 mL 酚类化合物，即水提取物的三倍多。根据DPPH中心点减少，乙醇提取物的抗氧化活性高出2.3倍（P
The aim of this study was to investigate antiradical activity of aqueous and ethanolic hawthorn fruit extracts, their flavonoids, and flavonoid combinations.Material and methods. Total amount of phenolic compounds and the constituents of flavonoids were determined using a high-performance liquid chromatography. The antioxidant activity of Crataegus monogyna extracts and flavonoids (chlorogenic acid, hyperoside, rutin, quercetin, vitexin-2O-rhamnoside, epicatechin, catechin, and procyanidin B-2) quantitatively was determined using the method of spectrophotometry (diphenyl-1-picrylhydrazyl (DPPH center dot) radical scavenging assay and 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid)(ABTS center dot+) radical cation decolorization assay). The level of tyrosine nitration inhibition was determined using a high-performance liquid chromatography.Results. Ethanolic hawthorn fruit extract contained 182 +/- 4 mg/100 mL phenolic compounds, i.e. threefold more, as compared to aqueous extract. The antioxidant activity according to DPPH center dot reduction in the ethanolic extracts was higher 2.3 times (P