Constitutive Expression of a miR319 Gene Alters Plant Development and Enhances Salt and Drought Tolerance in Transgenic Creeping Bentgrass1[W][OA]
Constitutive Expression of a miR319 Gene Alters Plant Development and Enhances Salt and Drought Tolerance in Transgenic Creeping Bentgrass1[W][OA]
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DOI：
10.1104/pp.112.208702
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发表时间：
2013-01
期刊：
影响因子：
7.4
通讯作者：
Man Zhou;Dayong Li;Zhigang Li;Q. Hu;Chunhua Yang;Lihuang Zhu;Hong Luo
中科院分区：
文献类型：
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作者：
Man Zhou;Dayong Li;Zhigang Li;Q. Hu;Chunhua Yang;Lihuang Zhu;Hong Luo
MiRNA 319影响植物对干旱和盐胁迫的响应，并且可以在转基因植物中进行操作以增强环境胁迫下的性能。MicroRNA 319（miR 319）是植物中最早被鉴定和保守的microRNA家族之一，并且已被证明靶向编码植物特异性转录因子的TCP（TEOSINTE BRANCHED/CYCLOIDEA/PROLIFERATING CELL FACTORS [PCF]）基因。MiR 319的表达受环境刺激的调节，这表明它参与了植物的胁迫反应，尽管缺乏实验证据，其潜在的机制仍然难以捉摸。本研究利用过表达水稻miR 319基因Osa-miR 319 a的转基因匍匐翦股颖（Agrostis stolonifera）研究miR 319在植物对非生物胁迫响应中的作用。我们发现过表达Osa-miR 319 a的转基因植物表现出形态学变化，并表现出增强的耐旱性和耐盐性，这与叶蜡含量和保水性增加有关，但钠吸收减少。基因表达分析表明，至少有四个推定的miR 319靶基因，AsPCF 5，AsPCF 6，AsPCF 8和AsTCP 14，以及水稻NAC结构域基因AsNAC 60的同源物在转基因植物中下调。我们的研究结果表明miR 319控制植物对干旱和盐胁迫的响应。转基因植物中增强的非生物胁迫耐受性与miR 319靶基因的显著下调有关，这意味着它们在开发新的分子策略以遗传工程改造作物物种以增强对环境胁迫的抗性中的潜力。
MiRNA319 affects plant responses to drought and salinity stress and can be manipulated in transgenic plants to enhance performance under environmental stress. MicroRNA319 (miR319) is one of the first characterized and conserved microRNA families in plants and has been demonstrated to target TCP (for TEOSINTE BRANCHED/CYCLOIDEA/PROLIFERATING CELL FACTORS [PCF]) genes encoding plant-specific transcription factors. MiR319 expression is regulated by environmental stimuli, suggesting its involvement in plant stress response, although experimental evidence is lacking and the underlying mechanism remains elusive. This study investigates the role that miR319 plays in the plant response to abiotic stress using transgenic creeping bentgrass (Agrostis stolonifera) overexpressing a rice (Oryza sativa) miR319 gene, Osa-miR319a. We found that transgenic plants overexpressing Osa-miR319a displayed morphological changes and exhibited enhanced drought and salt tolerance associated with increased leaf wax content and water retention but reduced sodium uptake. Gene expression analysis indicated that at least four putative miR319 target genes, AsPCF5, AsPCF6, AsPCF8, and AsTCP14, and a homolog of the rice NAC domain gene AsNAC60 were down-regulated in transgenic plants. Our results demonstrate that miR319 controls plant responses to drought and salinity stress. The enhanced abiotic stress tolerance in transgenic plants is related to significant down-regulation of miR319 target genes, implying their potential for use in the development of novel molecular strategies to genetically engineer crop species for enhanced resistance to environmental stress.