Characterization of an iridovirus detected from cultured turbot Scophthalmus maximus in Korea
Characterization of an iridovirus detected from cultured turbot Scophthalmus maximus in Korea
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DOI：
10.3354/dao064175
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发表时间：
2005-04-18
影响因子：
1.4
通讯作者：
Kitamura, SI
中科院分区：
文献类型：
--
作者：
Kim, WS;Oh, MJ;Kitamura, SI
2003年，在韩国高昌海水养殖场发生的一次疾病爆发中，大菱鲆幼鱼患病，对该幼鱼进行了检查，以确定疾病的原因。这条鱼的体色苍白，腹部增大，眼睛突出，脾脏和肾脏增大，鳃和/或肝脏苍白。组织学检查显示肾脏、脾脏、鳃、心脏、胃、肠、肝脏、胰腺和皮肤中的嗜碱性增大细胞。在扩大的细胞中观察到直径为136至159 nm的病毒颗粒。利用PCR技术扩增了大菱鲆虹彩病毒（TBIV）主要衣壳蛋白（MCP）基因的特异性片段。TBIV的MCP基因序列与虹彩病毒科热带病毒属（Tropivirus）的5种病毒（红鲷虹彩病毒、鲈鱼虹彩病毒、石斑鱼昏睡病虹彩病毒、非洲七鳃鳗虹彩病毒和侏儒鱼虹彩病毒）的MCP基因序列同源性大于93.76%。这些结果表明，从大菱鲆中检测到的病毒与拟议的热带病毒属相似。
Juvenile turbot Scophthalmus maximus that became sick during an outbreak of disease at mariculture facilities at Go-Chang, Korea, in 2003, were examined to identify the cause of the disease. The fish had pale body color, an enlarged abdomen, protruding eyes, an enlarged spleen and kidney, and pale gills and/or liver. Histopathogical examination revealed basophilic enlarged cells in the kidney, spleen, gills, heart, stomach, intestine, liver, pancreas and skin. Hexagonal viral particles with a diameter of 136 to 159 nm were observed in the enlarged cells. A specific 1299 bp fragment of the major capsid protein (MCP) gene of the turbot iridovirus (TBIV) was amplified by PCR. Sequence homology was greater than 93.76% between the MCP gene in TBIV and the same gene in 5 viruses in the tentatively proposed genus Tropivirus (family Iridoviridae): red sea bream iridovirus, sea bass iridovirus, grouper sleepy disease iridovirus, African lampeye iridovirus and dwarf gourami iridovirus. These results suggest that the virus detected from turbot is similar to the proposed genus Tropivirus.