Smad 3 regulates proliferation of the mouse ovarian surface epithelium.
Smad 3 regulates proliferation of the mouse ovarian surface epithelium.
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DOI：
10.1002/ar.a.10090
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发表时间：
2003-08
期刊：
影响因子：
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通讯作者：
D. Symonds;D. Tomić;Christina Borgeest;E. Mcgee;J. Flaws
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文献类型：
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作者：
D. Symonds;D. Tomić;Christina Borgeest;E. Mcgee;J. Flaws
Smad 3是转化生长因子β（TGF β）家族的信号中间体;然而，关于该蛋白在卵巢表面上皮（OSE）的调节中所起的作用知之甚少。使用转基因小鼠模型，我们发现，在Smad 3的情况下，OSE细胞有一个明显的形态学改变。野生型（WT）OSE是扁平的，细胞薄，而Smad 3缺陷型（Smad 3 -/-）OSE是厚的，细胞饱满立方。WT OSE对增殖细胞核抗原（PCNA）和雌激素受体α（ER α）的免疫染色比Smad 3 -/- OSE少。然而，WT和Smad 3 -/- OSE之间的凋亡细胞数量或Bax和Bcl-2水平没有差异。尽管WT小鼠的血清雌二醇水平高于Smad 3 -/-小鼠，但WT和Smad 3 -/-小鼠的孕酮水平相似。这些数据表明，在血清雌二醇水平不高或孕酮水平不改变的情况下，Smad 3调节OSE的形态外观和增殖。
Smad 3 is a signaling intermediate for the transforming growth factor beta (TGFbeta) family; however, little is known about the role this protein plays in the regulation of the ovarian surface epithelium (OSE). Using a transgenic mouse model, we found that in the absence of Smad 3 there was a distinct morphological alteration of OSE cells. Wild-type (WT) OSE was flat with thin cells, while Smad 3-deficient (Smad 3 -/-) OSE was thick with plump cuboidal cells. WT OSE had less immunostaining for proliferating cell nuclear antigen (PCNA) and estrogen receptor alpha (ERalpha) than Smad 3 -/- OSE. However, there were no differences in the number of apoptotic cells or Bax and Bcl-2 levels between WT and Smad 3 -/- OSE. Although WT mice had higher levels of serum estradiol than Smad 3 -/- mice, WT and Smad 3 -/- mice had similar levels of progesterone. These data suggest that Smad 3 regulates OSE morphological appearance and proliferation in the absence of high serum estradiol levels or alterations in progesterone levels.