A proteome analysis of the anterior cingulate cortex gray matter in schizophrenia
A proteome analysis of the anterior cingulate cortex gray matter in schizophrenia
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DOI：
10.1038/sj.mp.4001806
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发表时间：
2006-05-01
影响因子：
11
通讯作者：
Matsumoto, I
中科院分区：
文献类型：
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作者：
Clark, D;Dedova, I;Matsumoto, I
前扣带皮层（ACC，Brodmans区24）涉及精神分裂症的发病机制，这是由于其正常功能和连接性以及疾病状态下该脑区域内的结构、形态和神经递质畸变的报道。应用双向凝胶电泳（2DE）技术对精神分裂症患者（n = 10）和正常对照组（n = 10）尸体脑组织ACC灰质蛋白质组进行了扫描和比较。这种蛋白质组学方法在精神分裂症队列中检测到42个水平改变的蛋白质点，据我们所知，这是精神分裂症ACC的第一个蛋白质组学分析。这些蛋白质中的39个随后使用质谱法鉴定，并在功能上分为代谢和氧化应激、细胞骨架、突触、信号传导、运输和神经胶质特异性组。一些已鉴定的蛋白质先前已与疾病的发病机制有关，一些蛋白质为精神分裂症提供了新的见解。研究这些蛋白质，编码这些蛋白质的基因，它们的功能和相互作用可能揭示精神分裂症异质性症状特征的分子机制。
The Anterior Cingulate Cortex (ACC, Brodmans Area 24) is implicated in the pathogenesis of schizophrenia due to its normal functions and connectivity together with reports of structural, morphological and neurotransmitter aberrations within this brain area in the disease state. Two-dimensional gel electrophoresis (2DE) was employed to scan and compare the ACC gray matter proteomes between schizophrenia (n = 10) and control (n = 10) post-mortem human tissue. This proteomic approach has detected 42 protein spots with altered levels in the schizophrenia cohort, which to our knowledge is the first proteomic analysis of the ACC in schizophrenia. Thirty nine of these proteins were subsequently identified using mass spectrometry and functionally classified into metabolism and oxidative stress, cytoskeletal, synaptic, signalling, trafficking and glial-specific groups. Some of the identified proteins have previously been implicated in the disease pathogenesis and some offer new insights into schizophrenia. Investigating these proteins, the genes encoding these proteins, their functions and interactions may shed light on the molecular mechanisms underlying the heterogeneous symptoms characteristic of schizophrenia.