Phospholipid solubility determined by equilibrium distribution between surface and bulk phases
Phospholipid solubility determined by equilibrium distribution between surface and bulk phases
复制标题
DOI：
10.1021/la047086k
复制
发表时间：
2005-07-05
期刊：
影响因子：
3.9
通讯作者：
Feigenson, GW
中科院分区：
文献类型：
--
作者：
Buboltz, JT;Feigenson, GW
提出了一种确定双层形成磷脂的极低水溶解度极限的一般策略。该策略利用磷脂固有的表面活性，并被称为EDSB，代表表面和本体相之间的平衡分布。在这份报告中，EDSB已被用来确定临界双分子层浓度的二月桂酰磷脂酰胆碱（DLPC），短链双分子层形成磷脂。室温下，在中性pH缓冲液中，CBCDLPC = 2.5 x 10（-8）M。使用摩尔分数浓度标度，这对应于DLPC双层膜形成的-12.8kcal/mol的标准状态自由能变化。
A general strategy is proposed for determining the very low aqueous solubility limits of bilayer-forming phospholipids. The strategy exploits the inherent surface activity of phospholipids and has been termed EDSB, which stands for Equilibrium Distribution between Surface and Bulk phases. In this report, EDSB has been used to determine the critical bilayer concentration of dilauroylphosphatidylycholine (DLPC), a short-chain bilayer-forming phospholipid. At room temperature in neutral pH buffer, CBCDLPC = 2.5 x 10(-8) M. Using a mole fraction concentration scale, this corresponds to a standard-state free energy change of -12.8 kcal/mol for DLPC bilayer membrane formation.