A near-infrared fluorescent probe for rapid and selective detection of hydrosulfide and imaging in live cells
A near-infrared fluorescent probe for rapid and selective detection of hydrosulfide and imaging in live cells
复制标题
一种近红外荧光探针，用于快速、选择性地检测活细胞中的硫氢化物和成像
DOI：
10.1007/s11164-016-2805-3
复制
发表时间：
2017-05-01
影响因子：
3.3
通讯作者：
Chen, Ligong
中科院分区：
文献类型：
--
作者：
Jiang, Yichen;Jin, Di;Chen, Ligong
A heptamethine cyanine-based near-infrared (NIR) fluorescent probe Cy-1 for hydrosulfide detection was developed, with the response mechanism based on nucleophilic substitution of acryloyl moiety by HS-. Upon addition of NaHS, Cy-1 displayed a rapid fluorescence quenching (excited at 700 nm) and a quick fluorescence enhancement (excited at 544 nm) as well as obvious changes in absorption spectra and color. The response was fast within tens of seconds and could be observed by naked eyes. By contrasting Cy-1 with the reported probe CyAC, the superiority of Cy-1 in selectivity over Cys was demonstrated. Besides, it also exhibited excellent selectivity and sensitivity to HS- over various anions (0.33-mu M detection limit). Additionally, this probe was successfully applied in fetal bovine serum samples and imaging of hydrosulfide in living cells.
