Construction of thermotolerant yeast expressing thermostable cellulase genes
Construction of thermotolerant yeast expressing thermostable cellulase genes
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DOI：
10.1016/j.jbiotec.2007.03.008
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发表时间：
2007-06-15
影响因子：
4.1
通讯作者：
Tamaki, Hisanori
中科院分区：
文献类型：
--
作者：
Hong, Jiong;Wang, Yonghong;Tamaki, Hisanori
马克斯克鲁维酵母NBRC 1777被鉴定为耐热酵母，并被开发作为表达耐热纤维素酶基因的宿主。将先前分离的耐热内切β-1，4-葡聚糖酶、细胞生物水解酶和β-葡糖苷酶基因导入K. marxianus中表达，并在高表达启动子的控制下成功表达。重组K.表达纤维素酶基因的Marxianus能够在含有纤维二糖或羧甲基纤维素作为单一碳源的合成培养基中生长。重组菌发酵10%纤维二糖产乙醇43.4g/L。这些结果表明K. marxianus可能提供一个有用的宿主系统，可用于同时糖化发酵和纤维素强化生物加工的基础。(c)2007 Elsevier B. V.保留所有权利。
Kluyveromyces marxianus NBRC 1777 was identified as a thermotolerant yeast and was developed as a host for the expression of thermostable cellulase genes. The previously isolated genes for thermostable endo-beta-1,4-glucanase, cell obiohydrolase, and P-glucosidase were introduced into the chromosome of K. marxianus and successfully expressed under the control of high-expression promoters. The recombinant K. marxianus expressing cellulase genes became able to grow in synthetic medium containing cellobiose or carboxymethyl-cellulose as the single carbon source. Moreover, the recombinant strain produced 43.4 g/L ethanol from 10% cellobiose. These results suggest that K. marxianus may afford a useful host system, which may be applicable to the simultaneous saccharification and fermentation and the foundation of cellulose consolidated bioprocessing. (c) 2007 Elsevier B.V. All rights reserved.