RES2(+), A NEW MEMBER OF THE CDC10(+)/SWI4 FAMILY, CONTROLS THE START OF MITOTIC AND MEIOTIC CYCLES IN FISSION YEAST
RES2(+), A NEW MEMBER OF THE CDC10(+)/SWI4 FAMILY, CONTROLS THE START OF MITOTIC AND MEIOTIC CYCLES IN FISSION YEAST
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DOI：
10.1002/j.1460-2075.1994.tb06456.x
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发表时间：
1994-04-15
期刊：
影响因子：
11.4
通讯作者：
OKAYAMA, H
中科院分区：
文献类型：
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作者：
MIYAMOTO, M;TANAKA, K;OKAYAMA, H
在裂殖酵母中，cdc10(+)和res1(+)基因在有丝分裂和减数分裂周期的启动中起着至关重要的作用。它们编码结构相关的转录复杂蛋白，并调控一些S阶段特有的基因。在这里，我们报告了该家族的一个新成员的鉴定，命名为res2(+)。Res2(+)已被分离为一个res1(-)缺失突变体的多拷贝抑制因子，它指定了一个73 kDa的蛋白，它有两个Swi/ankyrin蛾的拷贝，与res1蛋白有最高的序列和结构相似性。Res2(+)与res1(+)在功能上是冗余的，是启动有丝分裂和减数分裂前DNA合成所必需的，但在减数分裂中具有额外的作用。与res1(+)不同，res2(+)在结合过程中被高度诱导，并且强烈依赖于cdc10(+)的活性。我们认为，裂殖酵母含有两个功能重叠的平行“起始”系统，即res1-cdc10和res2-cdc10，前者在有丝分裂周期中起主要作用，而后者在减数分裂周期中起主要作用。
In the fission yeast Schizosaccharomyces pombe, the cdc10(+) and res1(+) genes play a crucial role in the start of mitotic and meiotic cycles. They encode structurally related transcriptional complex proteins and regulate some S phase-specific genes. Here we report the identification of a new member of this family named as res2(+). res2(+) has been isolated as a multicopy suppressor of a res1(-) null mutant and specifies a 73 kDa protein, which has two copies of the Swi/ankyrin moth and shares the highest sequence and structure similarity with the Res1 protein. res2(+) is largely redundant in function with res1(+) and is required for the initiation of mitotic and premeiotic DNA synthesis, but has an additional role in meiotic division. Unlike res1(+), res2(+) is highly induced during conjugation and strongly depends on cdc10(+) for its activity. We conclude that the fission yeast contains two functionally overlapping parallel 'start' systems, Res1-Cdc10 and Res2-Cdc10, the former of which plays a major role in mitotic cycle whereas the latter in meiotic cycle.