Unveiling the Biosynthetic Pathway of the Ribosomally Synthesized and Post-translationally Modified Peptide Ustiloxin B in Filamentous Fungi.
Unveiling the Biosynthetic Pathway of the Ribosomally Synthesized and Post-translationally Modified Peptide Ustiloxin B in Filamentous Fungi.
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DOI：
10.1002/anie.201602611
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发表时间：
2016-07
影响因子：
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通讯作者：
Y. Ye;A. Minami;Y. Igarashi;M. Izumikawa;Myco Umemura;N. Nagano;M. Machida;Teppei Kawahara;K. Shin‐ya;K. Gomi;H. Oikawa
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文献类型：
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作者：
Y. Ye;A. Minami;Y. Igarashi;M. Izumikawa;Myco Umemura;N. Nagano;M. Machida;Teppei Kawahara;K. Shin‐ya;K. Gomi;H. Oikawa
通过一系列基因失活和异源表达研究，阐明了第一个真菌核糖体合成和后修饰肽（RiPP）ustiloxin B的生物合成机制。结果证实了具有DUF 3328基序的新型氧化酶用于大环化的基本要求，以及三种氧化酶（UstCF 1F 2）和吡哆醛5 '-磷酸（PLP）依赖性酶（UstD）的高度独特的侧链修饰。这些发现为在各种真菌中发现的RiPP基因簇的表达提供了新的见解。
The biosynthetic machinery of the first fungal ribosomally synthesized and post-translationally modified peptide (RiPP) ustiloxin B was elucidated through a series of gene inactivation and heterologous expression studies. The results confirmed an essential requirement for novel oxidases possessing the DUF3328 motif for macrocyclization, and highly unique side-chain modifications by three oxidases (UstCF1F2) and a pyridoxal 5'-phosphate (PLP)-dependent enzyme (UstD). These findings provide new insight into the expression of the RiPP gene clusters found in various fungi.