Immunohistochemical identification of intracytoplasmic lumens by cytokeratin typing may differentiate renal oncocytomas from chromophobe renal cell carcinomas.
Immunohistochemical identification of intracytoplasmic lumens by cytokeratin typing may differentiate renal oncocytomas from chromophobe renal cell carcinomas.
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DOI：
10.14670/hh-19.23
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发表时间：
2004
影响因子：
2
通讯作者：
Naoto Kuroda;M. Toi;M. Yamamoto;E. Miyazaki;Yoshihiro Hayashi;M. Hiroi;Taro Shuin;H. Enzan
中科院分区：
文献类型：
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作者：
Naoto Kuroda;M. Toi;M. Yamamoto;E. Miyazaki;Yoshihiro Hayashi;M. Hiroi;Taro Shuin;H. Enzan
肾嗜酸细胞瘤和嫌色肾细胞癌 (RCC) 具有共同的表型，均起源于集合管的嵌入细胞。这使得通过免疫组织化学方法区分两种肿瘤非常困难。因此，我们研究了免疫组织化学的结果，重点关注肾嗜酸细胞瘤中偶尔存在的某些特征结构。我们对 4 个嗜酸细胞瘤和 6 个嫌色细胞 RCC 中的各种细胞角蛋白（细胞角蛋白 7、8、10、10/13、14、18、19 和 20 以及 AE1/AE3）进行了 Hale 胶体铁染色和免疫组织化学分析。此外，还使用电子显微镜研究了一种肾嗜酸细胞瘤和一种嫌色肾细胞癌。两个肾嗜酸细胞瘤和一个嫌色肾细胞癌完全未被胶体铁染色。嗜酸细胞瘤和嫌色肾细胞癌的免疫组织化学特征没有明显差异。然而，在所有四种肾嗜酸细胞瘤中，我们都发现了一些细胞角蛋白（至少细胞角蛋白7、8和19以及AE1/AE3的3种抗原）的胞浆内环状阳性反应，其在超微结构上对应于胞浆内腔（ICL）。相比之下，使用所用抗体在任何嫌色体 RCC 中均未发现此类结构。因此，通过细胞角蛋白分型对 ICL 进行免疫组织化学鉴定可能有助于区分肾嗜酸细胞瘤和嫌色 RCC，并且在这方面比胶体铁染色更敏感。
Renal oncocytomas and chromophobe renal cell carcinomas (RCCs) share a common phenotype and both originate from the intercalated cells of the collecting duct. This makes it very difficult to differentiate between the two tumors immunohistochemically. Therefore, we studied the results of immunohistochemistry focusing on certain characteristic structures that are occasionally present in renal oncocytomas. We carried out Hale's colloidal iron staining and immunohistochemistry for various cytokeratins (cytokeratins 7, 8, 10, 10/13, 14, 18, 19 and 20, and AE1/AE3) in four oncocytomas and six chromophobe RCCs. In addition, one renal oncocytoma and one chromophobe RCC were studied using electron microscopy. Two renal oncocytomas and one chromophobe RCC were completely unstained by colloidal iron. There was no evident difference between the immunohistochemical characteristics of oncocytomas and those of chromophobe RCCs. However, in all four renal oncocytomas we identified intracytoplasmic ring-like positive reactions for some cytokeratins (at least 3 antigens of cytokeratins 7, 8 and 19, and AE1/AE3), which corresponded ultrastructurally to the intracytoplasmic lumens (ICLs). In contrast, no such structures were found in any of the chromophobe RCCs using the antibodies employed. Therefore, immunohistochemical identification of ICLs by cytokeratin typing may be useful for differentiating between renal oncocytomas and chromophobe RCCs and be more sensitive in this respect than colloidal iron staining.