A method for the design of 3D scaffolds for high-density cell attachment and determination of optimum perfusion culture conditions
A method for the design of 3D scaffolds for high-density cell attachment and determination of optimum perfusion culture conditions
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DOI：
10.1016/j.jbiomech.2008.02.025
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发表时间：
2008-01-01
影响因子：
2.4
通讯作者：
Shirakashi, Ryo
中科院分区：
文献类型：
--
作者：
Provin, Christophe;Takano, Kiyoshi;Shirakashi, Ryo
体外培养细胞在组织工程或药物筛选中的应用，针对的是肝脏等复杂的软组织，需要培养细胞具有体内的生理功能。为此，培养细胞的支架应提供类似于体内微环境，具有三维细胞外基质、高O-2和营养物质供应能力以及高细胞密度。在本文中，我们提出了一种设计方法（1）支架的几何形状，优化表面/体积比以允许高密度（5 x 10(7) 细胞/mL）细胞培养和（2）提供最佳数量的氧气和营养物质的培养条件。使用质量传递的 CFD 模型来确定各种流速下支架（20 毫米宽 - 35 毫米长）中的剪切应力以及 0 和葡萄糖代谢。通过与流阻和微型 PIV 实验的比较来验证模型。 CFD分析显示，该支架的最大代谢率密度为0.71 mL/min的O-2为6.04 x 10(-3) mol/s/m(3)，0.35 mL/min的葡萄糖的最大代谢率密度为1.91 X 10(-2) mol/s/m(3)。 (c) 2008 Elsevier Ltd. 保留所有权利。
The application of in vitro cultured cells in tissue engineering or drug screening, aimed at complex soft tissues such as liver, requires in vivo physiological function of the cultured cells. For this purpose, the scaffold in which cells are cultured should provide a microenvironment similar to an in vivo one with a three-dimensional extracellular matrix, a high supply capacity of O-2 and nutrients, and high cell density. In this paper, we propose a method to design (1) the geometry of the scaffold, with a surface/volume ratio optimized to allow high-density (5 x 10(7) cells/mL) cell culture and (2) culture conditions that will supply optimal quantities of oxygen and nutrients. CFD modeling of mass transport was used to determine the shear stress as well as 0, and glucose metabolism in the scaffold (20mm width-35mm length) for various flow rates. Validation of the model was done through comparison with flow resistance and micro-PIV experiments. CFD analysis showed the maximum metabolic rate densities for this scaffold are 6.04 x 10(-3) mol/s/m(3) for O-2 at 0.71 mL/min and 1.91 X 10(-2) mol/s/m(3) for glucose at 0.35 mL/min. (c) 2008 Elsevier Ltd. All rights reserved.