A picoliter 'fountain-pen' using co-axial dual pipettes
A picoliter 'fountain-pen' using co-axial dual pipettes
复制标题
DOI：
10.1016/s0165-0270(03)00099-2
复制
发表时间：
2003-07-15
影响因子：
3
通讯作者：
Moses, E
中科院分区：
文献类型：
--
作者：
Feinerman, O;Moses, E
介绍了一种用于局部可控药物输注的双移液管系统。两个同心移液器可以单独操作，并通过指定的双支架独立加压。内移液器。内层装载所需的溶液（药物），作为源，而外层是汇。这使得溶液在两个移液器之间流动，只突出一小段距离进入周围的流体，而不会扩散出去。输液时间分辨率可控，可产生振荡流。演示了三种双移液器系统的实现。我们证明了神经递质在神经网络中的局部应用是刺激网络活动的有效方法。然后，我们提出了一种湿微光刻技术，使用局部应用蛋白质到底物上。最后，我们展示了我们可以将给定的药物定位在细胞的一个小目标部分上。(C) 2003 Elsevier Science B.V.版权所有
A double pipette system for local, controlled drug infusion is presented. Two concentric pipettes can be manipulated separately and pressurized independently by a designated double holder. The inner pipette. is loaded with the desirable solution (drug), and functions as a source, while the outer one is a sink. This gives a flow of the solution between the two pipettes that protrudes only a small distance into the surrounding fluid and does not diffuse away. Time resolution of the infusion is highly controllable, and oscillatory flow can be generated. Three implementations of the double pipette system are demonstrated. We show that local application of neurotransmitters in neuronal networks is an efficient way of stimulating activity in the network. We then present a wet micro lithography technique using topical application of proteins onto the substrate. Finally, we show that we can localize a given drug on a small targeted part of a cell. (C) 2003 Elsevier Science B.V. All rights reserved.