Locomotion in larval zebrafish: Influence of time of day, lighting and ethanol
Locomotion in larval zebrafish: Influence of time of day, lighting and ethanol
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DOI：
10.1016/j.neuro.2008.09.011
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发表时间：
2009-01-01
期刊：
影响因子：
3.4
通讯作者：
Padilla, S.
中科院分区：
文献类型：
--
作者：
MacPhail, R. C.;Brooks, J.;Padilla, S.
斑马鱼(Danio Rerio)在发育研究中的使用越来越多，突显了对其行为进行详细了解的必要性。我们研究了斑马鱼幼体个体(受精后6天)在96孔板上的运动。移动是使用视频跟踪系统记录的。一天中的时间结果表明，在黑暗(红外线)中测试的活动从清晨开始逐渐减少到13：00到15：30小时之间的稳定水平。所有进一步的研究都在下午早些时候到晚些时候进行，持续大约1小时。每项研究也以一段黑暗时期开始，以最大限度地减少将平板转移到记录平台所造成的任何意外刺激。黑暗中的运动在4min时开始增加，到20min时逐渐下降到较低水平。光照时的活动量最初很低，20分钟后逐渐增加到一个稳定的水平。当光和暗交替10分钟时，活动在光中低，在暗中高；奇怪的是，交替暗期间的活动明显高于最初在延长的黑暗或光中获得的活动。进一步的实验探索了影响这种交替活动模式的变量。改变最初黑暗时期的持续时间(10-20分钟)不会影响随后在光明或黑暗中的活动。然而，返回黑暗时的活性增加在15分钟后比5分钟的光照下更大。急性乙醇在1%和2%时活性增加，在4%时活性严重降低。1%的乙醇延缓了活性从暗到光的转变，并延缓了暗中活性的习惯化，而2%的乙醇无论在什么光照条件下都能提高活性。总而言之，这些结果表明，斑马鱼幼体的运动量可以在96孔微滴定板格式中可靠地测量，并且对一天中的时间、光照条件和乙醇很敏感。由爱思唯尔公司出版。
The increasing use of zebrafish (Danio rerio) in developmental research highlights the need for a detailed understanding of their behavior. We studied the locomotion of individual zebrafish larva (6 days post-fertilization) in 96-well microtiter plates. Movement was recorded using a video-tracking system. Time of day results indicated locomotion, tested in darkness (infrared), decreased gradually from early morning to a stable level between 13:00 and 15:30 h. All further studies were conducted in early-to-late afternoon and lasted approximately 1 h. Each study also began with a period of darkness to minimize any unintended stimulation caused by transferring the plates to the recording platform. Locomotion in darkness increased initially to a maximum at 4 min, then decreased steadily to a low level by 20 min. Locomotion during light was initially low and then gradually increased to a stable level after 20 min. When 10-min periods of light and dark were alternated, activity was low in light and high in dark; curiously, activity during alternating dark periods was markedly higher than originally obtained during either extended dark or light. Further experiments explored the variables influencing this alternating pattern of activity. Varying the duration of the initial dark period (10-20 min) did not affect subsequent activity in either light or dark. The activity increase on return to dark was, however, greater following 15 min than 5 min of light. Acute ethanol increased activity at 1 and 2% and severely decreased activity at 4%. One-percent ethanol retarded the transition in activity from dark to light, and the habituation of activity in dark, while 2% ethanol increased activity regardless of lighting condition. Collectively, these results show that locomotion in larval zebrafish can be reliably measured in a 96-well microtiter plate format, and is sensitive to time of day, lighting conditions, and ethanol. Published by Elsevier Inc.