Antibody response in the parotid fluid and serum of Irus monkeys (Macaca fascicularis) after local immunization with Streptococcus mutans
Antibody response in the parotid fluid and serum of Irus monkeys (Macaca fascicularis) after local immunization with Streptococcus mutans
复制标题
变形链球菌局部免疫后伊鲁斯猴（Macaca fasciculis）腮腺液和血清中的抗体反应
DOI：
10.1128/iai.12.2.281-292.1975
复制
发表时间：
1975
影响因子：
3.1
通讯作者：
R. Genco
中科院分区：
文献类型：
--
作者：
F. Emmings;R. Evans;R. Genco
本文研究了食蟹猴腮腺唾液和血清中对变形链球菌两种途径局部免疫的抗体应答，并进行了一年以上的比较。使用特异性抗球蛋白偶联物通过间接免疫荧光染色滴定抗体并进行分类。通过酶抑制试验测定抗葡糖基转移酶活性。首先通过将福尔马林杀死的细菌细胞和细胞产物皮下注射到四个主要唾液腺附近来免疫动物。然后通过将抗原逆行滴注到腮腺导管中来免疫猴子。广泛的皮下局部免疫仅产生血清应答。腮腺导管免疫后，腮腺唾液中出现高滴度的免疫球蛋白A（伊加）抗体，沿着微量的免疫球蛋白G（IgG）和免疫球蛋白M（IgM）抗体，血清中存在高滴度的IgG抗体，沿着较低滴度的伊加和IgM。腮腺液中的伊加抗体通过双重免疫荧光染色显示与抗原决定簇相关，所述抗原决定簇与针对人分泌成分的抗血清交叉反应。腮腺液和血清中的滴度在免疫停止时急剧下降。该响应模式是可再现的。从S.在血清中发现了变形菌，但在腮腺液中检测到的相对较少。通过腮腺导管进行的广泛免疫导致腺体的短暂功能损害，如唾液流速降低所证明的。我们的结论是腮腺导管免疫可以刺激唾液分泌型伊加抗菌抗体反应的有效方法。
The antibody response of Macaca fascicularis in parotid saliva and serum to local immunization by two routes with Streptococcus mutans was studied and compared over 1 year. Antibodies were titrated and classified by indirect immunofluorescent staining using specific antiglobulin conjugates. Antiglucosyltransferase activity was assayed by an enzyme inhibition test. Animals were immunized first by injecting formalin-killed bacterial cells and cell products subcutaneously into the vicinity of the four major salivary glands. The monkeys were next immunized by retrograde instillation of antigen into the parotid duct. Extensive subcutaneous local immunization gave a serum response only. After parotid duct immunization, high titers of immunoglobulin A (IgA) antibody, along with traces of immunoglobulin G (IgG) immunoglobulin M (IgM) antibody, appeared in the parotid saliva, and in the serum high titers of IgG antibody were present along with lower titers of IgA and IgM. IgA antibodies in parotid fluid were shown by double immunofluorescent staining to be associated with antigenic determinants which cross-reacted with an antiserum directed to human secretory component. Titers in parotid fluids and sera fell sharply when immunization was stopped. This response pattern was reproducible. High concentrations of antibody capable of inhibiting glucosyltransferase prepared from S. mutans were found in the sera, but relatively little was detected in the parotid fluids. Extensive immunization via the parotid duct resulted in transient functional impairment of the gland, as evidenced by diminished salivary flow rates. We conclude that parotid ductal immunization can be an effective method for stimulating a salivary secretory IgA antibacterial antibody response.